NDLI: Flexibility of the Prograamme of Spore Coat Formation in Bacillus subtilis: Bypass of CotE Requirement by Over-Production of CotH

Content Provider	PubMed Central
Author	Isticato, Rachele Teja, Sirec Giglio, Rosa Loredana, Baccigalupi Rusciano, Giulia Pesce, Giuseppe Zito, Gianluigi Sasso, Antonio Felice, Maurilio De Ricca, Ezio
Editor	Driks, Adam
Copyright Year	2013
Abstract	Bacterial spores are surrounded by the coat, a multilayered shell that contributes in protecting the genome during stress conditions. In Bacillus subtilis, the model organism for spore formers, the coat is composed by about seventy different proteins, organized into four layers by the action of several regulatory proteins. A major component of this regulatory network, CotE, is needed to assemble the outer coat and develop spores fully resistant to lysozyme and able to germinate efficiently. Another regulator, CotH, is controlled by CotE and is present in low amounts both during sporulation and in mature spores. In spite of this CotH controls the assembly of at least nine outer coat proteins and cooperates with CotE in producing fully resistant and efficiently germinating spores. In order to improve our understanding of CotH role in spore formation, we over-produced CotH by placing its coding region under the control of a promoter stronger than its own promoter but with a similar timing of activity during sporulation. Over-production of CotH in an otherwise wild type strain did not cause any major effect, whereas in a cotE null background a partial recovery of the phenotypes associated to the cotE null mutation was observed. Western blot, fluorescence microscopy and Surface-Enhanced Raman Scattering spectroscopy data indicate that, in the absence of CotE, over-production of CotH allowed the formation of spores overall resembling wild type spores and carrying in their coat some CotE−/CotH-dependant proteins. Our results suggest that the B. subtilis spore differentiation programme is flexible, and that an increase in the amount of a regulatory protein can replace a missing partner and partially substitute its function in the assembly of the spore coat.
Related Links	http://dx.doi.org/10.1371/journal.pone.0074949
Starting Page	74949
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-09-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Correction: Flexibility of the Programme of Spore Coat Formation in Bacillus subtilis: Bypass of CotE Requirement by Over-Production of CotH

Architecture and Assembly of the Bacillus subtilis Spore Coat

Assembly Requirements and Role of CotH during Spore Coat Formation in Bacillus subtilis

The Direct Interaction between Two Morphogenetic Proteins Is Essential for Spore Coat Formation in Bacillus subtilis

Antagonistic Role of CotG and CotH on Spore Germination and Coat Formation in Bacillus subtilis

Bacillus subtilis spore coat assembly requires cotH gene expression.

Erratum. Bacillus subtilis spore coat assembly requires cotH gene expression.

Interactions among CotB, CotG, and CotH during Assembly of the Bacillus subtilis Spore Coat

CotE Binds to CotC and CotU and Mediates Their Interaction during Spore Coat Formation in Bacillus subtilis ▿

Flexibility of the Prograamme of Spore Coat Formation in Bacillus subtilis: Bypass of CotE Requirement by Over-Production of CotH

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Correction: Flexibility of the Programme of Spore Coat Formation in Bacillus subtilis: Bypass of CotE Requirement by Over-Production of CotH

Architecture and Assembly of the Bacillus subtilis Spore Coat

Assembly Requirements and Role of CotH during Spore Coat Formation in Bacillus subtilis

The Direct Interaction between Two Morphogenetic Proteins Is Essential for Spore Coat Formation in Bacillus subtilis

Antagonistic Role of CotG and CotH on Spore Germination and Coat Formation in Bacillus subtilis

Bacillus subtilis spore coat assembly requires cotH gene expression.

Erratum. Bacillus subtilis spore coat assembly requires cotH gene expression.

Interactions among CotB, CotG, and CotH during Assembly of the Bacillus subtilis Spore Coat

CotE Binds to CotC and CotU and Mediates Their Interaction during Spore Coat Formation in Bacillus subtilis ▿

Flexibility of the Prograamme of Spore Coat Formation in Bacillus subtilis: Bypass of CotE Requirement by Over-Production of CotH