NDLI: Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks

Content Provider	PubMed Central
Author	Mejean, Cecile O. Schaefer, Andrew W. Buck, Kenneth B. Kress, Holger Alla, Shundrovsky Merrill, Jason W. Dufresne, Eric R. Paul, Forscher
Editor	Kreplak, Laurent
Copyright Year	2013
Abstract	Adhesions are multi-molecular complexes that transmit forces generated by a cell’s acto-myosin networks to external substrates. While the physical properties of some of the individual components of adhesions have been carefully characterized, the mechanics of the coupling between the cytoskeleton and the adhesion site as a whole are just beginning to be revealed. We characterized the mechanics of nascent adhesions mediated by the immunoglobulin-family cell adhesion molecule apCAM, which is known to interact with actin filaments. Using simultaneous visualization of actin flow and quantification of forces transmitted to apCAM-coated beads restrained with an optical trap, we found that adhesions are dynamic structures capable of transmitting a wide range of forces. For forces in the picoNewton scale, the nascent adhesions’ mechanical properties are dominated by an elastic structure which can be reversibly deformed by up to 1 µm. Large reversible deformations rule out an interface between substrate and cytoskeleton that is dominated by a number of stiff molecular springs in parallel, and favor a compliant cross-linked network. Such a compliant structure may increase the lifetime of a nascent adhesion, facilitating signaling and reinforcement.
Related Links	http://dx.doi.org/10.1371/journal.pone.0073389
Starting Page	73389
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-09-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Complex Intramolecular Mechanics of G-actin — An Elastic Network Study

Dynamin2 Organizes Lamellipodial Actin Networks to Orchestrate Lamellar Actomyosin

Disentangling Membrane Dynamics and Cell Migration; Differential Influences of F-actin and Cell-Matrix Adhesions

Contribution of the LIM Domain and Nebulin-Repeats to the Interaction of Lasp-2 with Actin Filaments and Focal Adhesions

Contraction Mechanisms in Composite Active Actin Networks

Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin

A Mechanism for Adding the First Link in a Nascent Actin Filament Chain

α-Actinin and Filamin Cooperatively Enhance the Stiffness of Actin Filament Networks

Data Mechanics and Coupling Geometry on Binary Bipartite Networks

Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks

Similar Documents

Complex Intramolecular Mechanics of G-actin — An Elastic Network Study

Dynamin2 Organizes Lamellipodial Actin Networks to Orchestrate Lamellar Actomyosin

Disentangling Membrane Dynamics and Cell Migration; Differential Influences of F-actin and Cell-Matrix Adhesions

Contribution of the LIM Domain and Nebulin-Repeats to the Interaction of Lasp-2 with Actin Filaments and Focal Adhesions

Contraction Mechanisms in Composite Active Actin Networks

Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin

A Mechanism for Adding the First Link in a Nascent Actin Filament Chain

α-Actinin and Filamin Cooperatively Enhance the Stiffness of Actin Filament Networks

Data Mechanics and Coupling Geometry on Binary Bipartite Networks

Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks