NDLI: The Synthetic Compound Norcantharidin Induced Apoptosis in Mantle Cell Lymphoma In Vivo and In Vitro through the PI3K-Akt-NF- κ B Signaling Pathway

Content Provider	PubMed Central
Author	Lv, Hongyan Yan, Li Du, Hengfei Fang, Jie Song, Xiaoning Zhang, Jinqiao
Copyright Year	2013
Abstract	This study aimed to elucidate the antitumor activity of norcantharidin (NCTD) against human mantle cell lymphoma (MCL). Cell proliferation and apoptosis were examined by MTS and flow cytometry. Caspase-3, -8, and -9 activities were detected with a colorimetric caspase protease assay. Apoptotic proteins—including PARP, cyclin D1, Bcl-2 family proteins, XIAP, and cIAP I—were studied by western blot. The phosphoinositide 3 kinase (PI3K) inhibitor LY294002 was used to investigate the involvement of the PI3K/Akt signaling pathway. In vivo studies were performed using Z138 cell xenografts in nude mice. NCTD inhibited proliferation and induced apoptosis of Z138 and Mino cells, both in vitro and in vivo. PI3Kp110 α and p-Akt expressions were downregulated by NCTD treatment. NCTD downregulated NF- κ B activity by preventing NF- κ B phosphorylation and nuclear translocation. This effect was correlated with the suppression of NF- κ B-regulated gene products, such as cyclin D1, BAX, survivin, Bcl-2, XIAP, and cIAP. This phenomenon was blocked by the PI3K inhibitor LY294002. Our results demonstrated that NCTD can induce growth arrest and apoptosis in MCL cells and that the mechanism may involve the PI3K/Akt/NF- κ B signaling pathway. NCTD may have therapeutic and/or adjuvant therapeutic applications in the treatment of MCL.
Related Links	http://dx.doi.org/10.1155/2013/461487
Starting Page	461487
File Format	PDF
ISSN	17414288
e-ISSN	17414288
Journal	Evidence-based Complementary and Alternative Medicine : eCAM
Volume Number	2013
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2013-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Complementary and Alternative Medicine

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