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| Content Provider | PubMed Central |
|---|---|
| Author | Ravkov, Eugene V. Pavlov, Igor Y. Hanson, Kimberly E. Delgado, Julio C. |
| Copyright Year | 2012 |
| Abstract | Cytomegalovirus (CMV) infection is one of the most important infectious complications of transplantation. Monitoring CMV-specific CD8 T cell immunity is useful for predicting active CMV infection and for directing targeted antiviral therapy. In this study, we examined four basic parameters for validation of CMV-specific tetramer staining and peptide stimulation assays that cover five most frequent HLA class I alleles. We also examined the potential use of CMV-specific CD8+ T cell numbers and functional and cytolytic responses in two autologous HSCT recipients treated for multiple myeloma. The coefficient of variation (CV %) of the precision within assays was 3.1−24% for HLA-tetramer staining, 2.5−47% for IFN- γ , and 3.4−59.7% for CD107a/b production upon peptide stimulation. The precision between assays was 5−26% for tetramer staining, 4−24% for IFN- γ , and 5−48% for CD107a/b. The limit of detection was 0.1−0.23 cells/ μ L of blood for tetramer staining, 0−0.23 cell/ μ L for IFN- γ , and 0.11−0.98 cells/ μ L for CD107a/b. The assays were linear and specific. The reference interval with 95% confidence level was 0−18 cells/ μ L for tetramer staining, 0−2 cells/ μ L for IFN- γ , and 0–3 cells/ μ L for CD107a/b. Our results provide acceptable measures of test performance for CMV immune competence assays for the characterization of CD8+ T cell responses posttransplant measured in the absolute cell count per μ L of blood. |
| Related Links | http://dx.doi.org/10.1155/2012/451059 |
| Starting Page | 451059 |
| File Format | |
| ISSN | 17402522 |
| e-ISSN | 17402530 |
| Journal | Clinical and Developmental Immunology |
| Volume Number | 2012 |
| Language | English |
| Publisher | Hindawi Publishing Corporation |
| Publisher Date | 2012-01-01 |
| Access Restriction | Open |
| Rights Holder | Hindawi Publishing Corporation |
| Subject Keyword | Immunology Immunology and Allergy Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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