NDLI: APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells

Content Provider	PubMed Central
Author	Shinohara, Masanobu Io, Katsuhiro Shindo, Keisuke Matsui, Masashi Sakamoto, Takashi Tada, Kohei Kobayashi, Masayuki Kadowaki, Norimitsu Takaori-kondo, Akifumi
Copyright Year	2012
Abstract	Human APOBEC3 proteins play pivotal roles in intracellular defense against viral infection by catalyzing deamination of cytidine residues, leading to base substitutions in viral DNA. Activation-induced cytidine deaminase (AID), another member of the APOBEC family, is capable of editing immunoglobulin (Ig) and non-Ig genes, and aberrant expression of AID leads to tumorigenesis. However, it remains unclear whether APOBEC3 (A3) proteins affect stability of human genome. Here we demonstrate that both A3A and A3B can induce base substitutions into human genome as AID can. A3B is highly expressed in several lymphoma cells and somatic mutations occur in some oncogenes of the cells highly expressing A3B. Furthermore, transfection of A3B gene into lymphoma cells induces base substitutions in cMYC gene. These data suggest that aberrant expression of A3B can evoke genomic instability by inducing base substitutions into human genome, which might lead to tumorigenesis in human cells.
Related Links	http://dx.doi.org/10.1038/srep00806
Starting Page	806
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	2
Language	English
Publisher	Nature Publishing Group
Publisher Date	2012-11-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells

Title APOBEC 3 B can impair genomic stability by inducing basesubstitutions in genomic DNA in human cells

Mouse transgenes in human cells detect specific base substitutions.

Degradation of the cancer genomic DNA deaminase APOBEC3B by SIV Vif

Endogenous APOBEC3B Overexpression Constitutively Generates DNA Substitutions and Deletions in Myeloma Cells

Human Papillomavirus E6 Triggers Upregulation of the Antiviral and Cancer Genomic DNA Deaminase APOBEC3B

Replication of acetylaminofluorene-adducted plasmids in human cells: spectrum of base substitutions and evidence of excision repair.

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis.

APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells

Similar Documents

APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells

Title APOBEC 3 B can impair genomic stability by inducing basesubstitutions in genomic DNA in human cells

Mouse transgenes in human cells detect specific base substitutions.

Degradation of the cancer genomic DNA deaminase APOBEC3B by SIV Vif

Endogenous APOBEC3B Overexpression Constitutively Generates DNA Substitutions and Deletions in Myeloma Cells

Human Papillomavirus E6 Triggers Upregulation of the Antiviral and Cancer Genomic DNA Deaminase APOBEC3B

Replication of acetylaminofluorene-adducted plasmids in human cells: spectrum of base substitutions and evidence of excision repair.

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis.

APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells