NDLI: The Aspergillus nidulans Kinesin-3 Tail Is Necessary and Sufficient to Recognize Modified Microtubules

Content Provider	PubMed Central
Author	Seidel, Constanze Nadine, Zekert Fischer, Reinhard
Editor	Alfredo, Herrera-estrella
Copyright Year	2012
Abstract	Posttranslational microtubule modifications (PTMs) are numerous; however, the biochemical and cell biological roles of those modifications remain mostly an enigma. The Aspergillus nidulans kinesin-3 UncA uses preferably modified microtubules (MTs) as tracks for vesicle transportation. Here, we show that a positively charged region in the tail of UncA (amino acids 1316 to 1402) is necessary for the recognition of modified MTs. Chimeric proteins composed of the kinesin-1 motor domain and the UncA tail displayed the same specificity as UncA, suggesting that the UncA tail is sufficient to establish specificity. Interaction between the UncA tail and alpha-tubulin was shown using a yeast two-hybrid assay and in A. nidulans by bimolecular fluorescence complementation. This is the first demonstration of how a kinesin-3 motor protein distinguishes among different MT populations in fungal cells, and how specificity determination depends on the tail rather than the motor domain, as has been demonstrated for kinesin 1 in neuronal cells.
Related Links	http://dx.doi.org/10.1371/journal.pone.0030976
Starting Page	30976
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-02-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Transportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin

A Single Protofilament Is Sufficient to Support Unidirectional Walking of Dynein and Kinesin

The Aspergillus nidulans CENP-E kinesin KipA is able to dimerize and to move processively along microtubules

A Cytosine Methyltransferase Homologue Is Essential for Sexual Development in Aspergillus nidulans

Listeriolysin O Is Necessary and Sufficient to Induce Autophagy during Listeria monocytogenes Infection

An EB1-kinesin complex is sufficient to steer microtubule growth in vitro

Boosting Long-Term Memory via Wakeful Rest: Intentional Rehearsal Is Not Necessary, Consolidation Is Sufficient

The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans

The Spt-Ada-Gcn5 Acetyltransferase (SAGA) Complex in Aspergillus nidulans

The Aspergillus nidulans Kinesin-3 Tail Is Necessary and Sufficient to Recognize Modified Microtubules

Similar Documents

Transportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin

A Single Protofilament Is Sufficient to Support Unidirectional Walking of Dynein and Kinesin

The Aspergillus nidulans CENP-E kinesin KipA is able to dimerize and to move processively along microtubules

A Cytosine Methyltransferase Homologue Is Essential for Sexual Development in Aspergillus nidulans

Listeriolysin O Is Necessary and Sufficient to Induce Autophagy during Listeria monocytogenes Infection

An EB1-kinesin complex is sufficient to steer microtubule growth in vitro

Boosting Long-Term Memory via Wakeful Rest: Intentional Rehearsal Is Not Necessary, Consolidation Is Sufficient

The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans

The Spt-Ada-Gcn5 Acetyltransferase (SAGA) Complex in Aspergillus nidulans

The Aspergillus nidulans Kinesin-3 Tail Is Necessary and Sufficient to Recognize Modified Microtubules