NDLI: Novel Insights into the Downstream Pathways and Targets Controlled by Transcription Factors CREM in the Testis

Content Provider	PubMed Central
Author	Kosir, Rok Peter, Juvan Perse, Martina Budefeld, Tomaz Majdic, Gregor Fink, Martina Paolo, Sassone-corsi Rozman, Damjana
Editor	Tora, Laszlo
Copyright Year	2012
Abstract	The essential role of the Crem gene in normal sperm development is widely accepted and is confirmed by azoospermia in male mice lacking the Crem gene. The exact number of genes affected by Crem absence is not known, however a large difference has been observed recently between the estimated number of differentially expressed genes found in Crem knock-out (KO) mice compared to the number of gene loci bound by CREM. We therefore re-examined global gene expression in male mice lacking the Crem gene using whole genome transcriptome analysis with Affymetrix microarrays and compared the lists of differentially expressed genes from Crem−/− mice to a dataset of genes where binding of CREM was determined by Chip-seq. We determined the global effect of CREM on spermatogenesis as well as distinguished between primary and secondary effects of the CREM absence. We demonstrated that the absence of Crem deregulates over 4700 genes in KO testis. Among them are 101 genes associated with spermatogenesis 41 of which are bound by CREM and are deregulated in Crem KO testis. Absence of several of these genes in mouse models has proven their importance for normal spermatogenesis and male fertility. Our study showed that the absence of Crem plays a more important role on different aspects of spermatogenesis as estimated previously, with its impact ranging from apoptosis induction to deregulation of major circadian clock genes, steroidogenesis and the cell-cell junction dynamics. Several new genes important for normal spermatogenesis and fertility are down-regulated in KO testis and are therefore possible novel targets of CREM.
Related Links	http://dx.doi.org/10.1371/journal.pone.0031798
Starting Page	31798
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-02-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Novel Insights into the Downstream Pathways and Targets Controlled by Transcription Factors CREM in the Testis

Global Genome Analysis of the Downstream Binding Targets of Testis Determining Factor SRY and SOX9

Novel Insights into the Transcriptome of Dirofilaria immitis

Comparative Transcriptome Analysis of Shoots and Roots of TNG67 and TCN1 Rice Seedlings under Cold Stress and Following Subsequent Recovery: Insights into Metabolic Pathways, Phytohormones, and Transcription Factors

Downstream Targets of Lmo4 Are Modulated by Cisplatin in the Inner Ear of Wistar Rats

Novel Insights into the Bovine Polled Phenotype and Horn Ontogenesis in Bovidae

Novel Insights into DNA Methylation Features in Spermatozoa:Stability and Peculiarities

Novel Insights into the Ontogeny of Nestmate Recognition in Polistes Social Wasps

NPPB and ACAN, Two Novel SHOX2 Transcription Targets Implicated in Skeletal Development

Novel Insights into the Downstream Pathways and Targets Controlled by Transcription Factors CREM in the Testis

Similar Documents

Novel Insights into the Downstream Pathways and Targets Controlled by Transcription Factors CREM in the Testis

Global Genome Analysis of the Downstream Binding Targets of Testis Determining Factor SRY and SOX9

Novel Insights into the Transcriptome of Dirofilaria immitis

Comparative Transcriptome Analysis of Shoots and Roots of TNG67 and TCN1 Rice Seedlings under Cold Stress and Following Subsequent Recovery: Insights into Metabolic Pathways, Phytohormones, and Transcription Factors

Downstream Targets of Lmo4 Are Modulated by Cisplatin in the Inner Ear of Wistar Rats

Novel Insights into the Bovine Polled Phenotype and Horn Ontogenesis in Bovidae

Novel Insights into DNA Methylation Features in Spermatozoa:Stability and Peculiarities

Novel Insights into the Ontogeny of Nestmate Recognition in Polistes Social Wasps

NPPB and ACAN, Two Novel SHOX2 Transcription Targets Implicated in Skeletal Development

Novel Insights into the Downstream Pathways and Targets Controlled by Transcription Factors CREM in the Testis