NDLI: A Genome-Wide Collection of Mos1 Transposon Insertion Mutants for the C. elegans Research Community

Content Provider	PubMed Central
Author	Vallin, Elodie Gallagher, Joseph Granger, Laure Martin, Edwige Jérôme, Belougne Maurizio, Julien Duverger, Yohann Scaglione, Sarah Borrel, Caroline Elisabeth, Cortier Karima, Abouzid Carre-pierrat, Maité Gieseler, Kathrin Laurent, Ségalat Kuwabara, Patricia E. Ewbank, Jonathan J.
Editor	Todd, Lamitina
Copyright Year	2012
Abstract	Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We describe here the optimization of a semi-automated methodology for the construction of a substantial collection of Mos1 insertion mutant strains. At peak production, more than 5,000 strains were generated per month. These strains were then subject to molecular analysis, and more than 13,300 Mos1 insertions characterized. In addition to targeting directly more than 4,700 genes, these alleles represent the potential starting point for the engineered deletion of essentially all C. elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource.
Related Links	http://dx.doi.org/10.1371/journal.pone.0030482
Starting Page	30482
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-02-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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