NDLI: Chloride transport in microperfused interlobular ducts isolated from guinea-pig pancreas

Content Provider	PubMed Central
Author	Ishiguro, H. Naruse, S. Kitagawa, M. Mabuchi, T. Kondo, T. Hayakawa, T. Case, R. M. Steward, M. C.
Copyright Year	2002
Abstract	Isolated interlobular ducts from the guinea-pig pancreas secrete a HCO3 −-rich fluid in response to secretin. To determine the role of Cl− transporters in this process, intracellular Cl− concentration ([Cl−]i) was measured in ducts loaded with the Cl−-sensitive fluoroprobe, 6-methoxy-N-ethylquinolinium chloride (MEQ). [Cl−]i decreased when the luminal Cl− concentration was reduced. This effect was stimulated by forskolin, was not dependent on HCO3 − and was not inhibited by application of the anion channel/transporter inhibitor H2DIDS to the luminal membrane. It is therefore attributed to a cAMP-stimulated Cl− conductance, probably the cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel. [Cl−]i also decreased when the basolateral Cl− concentration was reduced. This effect was not stimulated by forskolin, was largely dependent on HCO3 − and was inhibited by basolateral H2DIDS. It is therefore mediated mainly by Cl−/HCO3 − exchange. With high Cl− and low HCO3 − concentrations in the lumen, steady-state [Cl−]i was 25-35 mm in unstimulated cells. Stimulation with forskolin caused [Cl−]i to increase by approximately 4 mm due to activation of the luminal anion exchanger. With low Cl− and high HCO3 − concentrations in the lumen to simulate physiological conditions, steady-state [Cl−]i was 10–15 mm in unstimulated cells. Upon stimulation with forskolin, [Cl−]i fell to approximately 7 mm due to increased Cl− efflux via the luminal conductance. We conclude that, during stimulation under physiological conditions, [Cl−]i decreases to very low levels in guinea-pig pancreatic duct cells, largely as a result of the limited capacity of the basolateral transporters for Cl− uptake. The resulting lack of competition from intracellular Cl− may therefore favour HCO3 − secretion via anion conductances in the luminal membrane, possibly CFTR.
Related Links	http://dx.doi.org/10.1113/jphysiol.2001.012490
Starting Page	175
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	539
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2002-02-15
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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