NDLI: Temperature effect on isometric tension is mediated by regulatory proteins tropomyosin and troponin in bovine myocardium

Content Provider	PubMed Central
Author	Fujita, Hideaki Kawai, Masataka
Copyright Year	2002
Abstract	The effect of temperature on isometric tension with and without the regulatory proteins tropomyosin and troponin was studied in bovine myocardium using a thin filament removal and reconstitution protocol. In control bovine myocardium, isometric tension increased linearly with temperature in the range 5–40 °C: isometric tension at 10 and 30 °C was 0.65 and 1.28 times that at 20 °C, respectively, with a Q 10 of about 1.4. In actin filament-reconstituted myocardium without regulatory proteins, the temperature effect on isometric tension was less: isometric tension at 10 and 30 °C was 0.96 and 1.17 times that at 20 °C, respectively, with a Q 10 of about 1.1. The temperature dependence of the apparent rate constants was studied using sinusoidal analysis. The temperature dependence of 2πb (rate constant of delayed tension phase) did not vary significantly with the regulatory proteins under the standard activating condition (5 mm MgATP, 8 mm Pi, 200 mm ionic strength, pCa 4.66, pH 7.00). Q 10 for 2πb in control and actin filament-reconstituted myocardium was 3.8 and 4.0, respectively. There were two phases to the temperature dependence of 2πc (rate constant of quick recovery). In control and thin filament-reconstituted myocardium, Q 10 for 2πc was approximately 5.5 in the low temperature range (≤ 25 °C) and 2.7 in the high temperature range (≥ 30 °C). In actin filament-reconstituted myocardium, Q 10 for 2πc was 8.5 in the low temperature range and 3.6 in the high temperature range. The above results demonstrate that regulatory proteins augment the temperature dependence of isometric tension, indicating that the regulatory proteins may modify the actomyosin interaction.
Related Links	http://dx.doi.org/10.1113/jphysiol.2001.013220
Ending Page	276
Page Count	10
Starting Page	267
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	539
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2002-02-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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