NDLI: Dual effect of blocking agents on Ca2+-activated Cl− currents in rabbit pulmonary artery smooth muscle cells

Content Provider	PubMed Central
Author	Piper, A. S. Large, W. A. Greenwood, I. A.
Copyright Year	2002
Abstract	The effects of the Cl− channel antagonists, niflumic acid (NFA), dichloro-diphenylamine 2-carboxylic acid (DCDPC) and diisothiocyanato-stilbene-2,2′-disulphonic acid (DIDS) on Ca2+-activated Cl− current (I Cl(Ca)) evoked by adding fixed intracellular calcium concentrations ([Ca2+]i) to the pipette solution were studied in rabbit pulmonary artery myocytes. With 250 and 500 nm [Ca2+]i bath application of NFA (100 μm) increased inward current at negative potentials, but inhibited outward current at positive potentials. On wash out of NFA, I Cl(Ca) was greatly enhanced at all potentials. When external Na+ ions were replaced by N-methyl-d-glucamine (NMDG+) NFA still enhanced I Cl(Ca) at negative potentials but the increase of I Cl(Ca) on wash out was blocked. When the mean reversal potential (E r) of I Cl(Ca) was shifted to negative potentials by replacing external Cl− with SCN−, NFA increased inward current but blocked outward current suggesting that the effect of NFA is dependent on current flow. Inclusion of NFA in the pipette solution had no effect on I Cl(Ca). Voltage jump experiments indicated that I Cl(Ca) displayed characteristic outward current relaxations at +70 mV and inward current relaxations at −80 mV that were abolished by NFA. DCDPC (100 μm) produced similar effects to NFA but 1 mm DIDS produced inhibition of I Cl(Ca) at both positive and negative potentials and there was no increase in current on wash out of DIDS. These results suggest that NFA and DCDPC, but not DIDS, simultaneously enhance and block I Cl(Ca) by binding to an external site, probably close to the mouth of the chloride channel.
Related Links	http://dx.doi.org/10.1113/jphysiol.2001.013270
Starting Page	119
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	539
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2002-02-15
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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