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| Content Provider | PubMed Central |
|---|---|
| Author | Ishiguro, H. Naruse, S. Steward, M. C. Kitagawa, M. Ko, S. B. H. Hayakawa, T. Case, R. M. |
| Copyright Year | 1998 |
| Abstract | Pancreatic HCO3 − and fluid secretion were studied by monitoring luminal pH (pHL) and luminal volume simultaneously in interlobular duct segments isolated from guinea-pig pancreas. The secretory rate and HCO3 − flux were estimated from fluorescence images obtained following microinjection of BCECF-dextran (70 kDa, 20 μM) into the duct lumen. Ducts filled initially with a Cl−-rich solution swelled steadily (2.0 nl min−1 mm−2) when HCO3 −2/CO was introduced, and the luminal pH increased to 8.08. When Cl− was replaced by glucuronate, spontaneous fluid secretion was reduced by 75 %, and pHL did not rise above 7.3. Cl−-dependent spontaneous secretion was largely blocked by luminal H2DIDS (500 μM). We conclude that, in unstimulated ducts, HCO3 − transport across the luminal membrane is probably mediated by Cl−-HCO3 − exchange. Secretin (10 nM) and forskolin (1 μM) both stimulated HCO3 − and fluid secretion. The final value of pHL (8.4) and the increase in secretory rate (1.5 nl min−1 mm−2) after secretin stimulation were unaffected by substitution of Cl−. The Cl−-independent component of secretin-evoked secretion was not affected by luminal H2DIDS. This suggests that a Cl−-independent mechanism provides the main pathway for luminal HCO3 − transport in secretin-stimulated ducts. Ducts filled initially with a HCO3 −-rich fluid (125 mM HCO3 −, 23 mM Cl−) secreted a Cl−-rich fluid while unstimulated. This became HCO3 −-rich when secretin was applied. Addition of H2DIDS and MIA (10 μM) to the bath reduced the secretory rate by 56 and 18 %, respectively. Applied together they completely blocked fluid secretion. We conclude that basolateral HCO3 − transport is mediated mainly by Na+-HCO3 − cotransport rather than by Na+-H+ exchange. |
| Related Links | http://dx.doi.org/10.1111/j.1469-7793.1998.407bh.x |
| Ending Page | 422 |
| Page Count | 16 |
| Starting Page | 407 |
| File Format | |
| ISSN | 00223751 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 2 |
| Volume Number | 511 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 1998-09-01 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Physiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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