NDLI: Induction of extrinsic and intrinsic apoptosis in cervical cancer cells by Momordica dioica mediated gold nanoparticles

Content Provider	IET Digital Library
Author	Naik, Lavudya Srinivas Devi, Chinnapaka Venkata Ramana
Abstract	Bio-fabrication of gold nanoparticles (AuNPs) has several advantages like biocompatibility, less toxicity, and eco-friendly in nature over their chemical and physical methods. Currently, the authors fabricated AuNPs using aqueous root extract of Momordica dioica (M. dioica) and explored their anticancer application with mechanistic approaches. Different biophysical techniques such as UV–visible spectroscopy, Fourier transform infrared, X-ray diffraction, transmission electron microscopy, selected area electron diffraction, and dynamic light scattering were employed for AuNPs characterisation. The synthesised AuNPs were mono-dispersed, crystalline in nature, anionic surface (−23.9 mV), and spherical particle of an average diameter of 9.4 nm. In addition, the AuNPs were stable in buffers solutions and also biocompatible towards normal human cells (human vascular endothelial cells and human lung cells). The AuNPs were exhibited anticancer activity against different cancer cell lines such as human breast cancer cells, human cervical cancer cells (HeLa) and human lung cancer cells. Further, the pro-apoptotic genes such as Bcl2 were down-regulated and BAX, Caspase-3, −8, and −9 were up-regulated in HeLa cells as compared to untreated cells. Annexin-V-FITC assay results showed that the AuNPs were induced apoptosis by accumulation of intracellular reactive oxygen species. To their knowledge, this is the first report on the synthesis of bioactive metal nanoparticles from M. dioica and it may open up new avenues in therapeutic applications.
Starting Page	172
Ending Page	179
Page Count	8
ISSN	17518741
Volume Number	14
e-ISSN	1751875X
Issue Number	Issue 2, Apr (2020)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-nbt/14/2
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2019.0075
Journal	IET Nanobiotechnology
Publisher Date	2019-12-10
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Annexin-V-FITC Assay AuNPs Characterisation BAX Bcl2 Bioactive Metal Nanoparticles Biochemistry Biofabrication Biomedical Material Biomembranes Biomolecular Interactions Biophysical Technique Cancer Cancer Cell Line Caspase-3 Caspase-8 Caspase-9 Cellular Biophysics Charge Transfer Complexes Drug Delivery System Drugs Electromagnetic Radiation Spectrometry (chemical Analysis) Electron Diffraction Electronic Structure And Spectra of MacroMolecule Extrinsic Apoptosis Fourier Transform Infrared Spectra Gold HeLa Cell Human Breast Cancer Cell Human Cervical Cancer Cell Human Lung Cancer Cell Human Vascular Endothelial Cell Infrared Molecular Spectra Interactions with Radiations at The Biomolecular Level Intracellular Reactive Oxygen Species Intrinsic Apoptosis Light Scattering Lung Macromolecular Configuration (bonds, Dimensions) Mediated Gold NanoParticle Molecular Biophysics Momordica Dioica Mediated Gold NanoParticle Nanofabrication Nanomedicine Nanoparticle Nanotechnology Application in Biomedicine Natural And Artificial Biomembranes Normal Human Cell Particle Size Patient Care And Treatment Patient Diagnostic Method And Instrumentation Physical Chemistry of Biomolecular Solutions And Condensed State Physical Method Physics of Subcellular Structure Proapoptotic Genes Selected Area Electron Diffraction Size 9.4 Nm Therapeutic Application Toxicology Transmission Electron Microscopy Tumours Ultraviolet Molecular Spectra Ultraviolet Spectra Untreated Cell UV-visible Spectroscopy Visible Molecular Spectra Visible Spectra Voltage -23.9 MV X-Ray Diffraction
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Nutratherapeutics approach against cancer: tomato‐mediated synthesised gold nanoparticles

Nutratherapeutics approach against cancer: tomato-mediated synthesised gold nanoparticles

Silver nanoparticles biosynthesised using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line

Silver nanoparticles biosynthesised using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line.

Photo-induced characteristics of azobenzene based gold nanoparticles

Spectroscopic and microscopic analyses of rod-shaped gold nanoparticles interacting with single-stranded DNA oligonucleotides

Synthesis of Gold Nanoparticles Using Cefopernazone as a Stabilizing Reagent and Its Application

Biological synthesis of metallic nanoparticles using algae

Role of catalytic protein and stabilising agents in the transformation of Ag ions to nanoparticles by Pseudomonas aeruginosa

Induction of extrinsic and intrinsic apoptosis in cervical cancer cells by Momordica dioica mediated gold nanoparticles

Similar Documents

Nutratherapeutics approach against cancer: tomato‐mediated synthesised gold nanoparticles

Nutratherapeutics approach against cancer: tomato-mediated synthesised gold nanoparticles

Silver nanoparticles biosynthesised using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line

Silver nanoparticles biosynthesised using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line.

Photo-induced characteristics of azobenzene based gold nanoparticles

Spectroscopic and microscopic analyses of rod-shaped gold nanoparticles interacting with single-stranded DNA oligonucleotides

Synthesis of Gold Nanoparticles Using Cefopernazone as a Stabilizing Reagent and Its Application

Biological synthesis of metallic nanoparticles using algae

Role of catalytic protein and stabilising agents in the transformation of Ag ions to nanoparticles by Pseudomonas aeruginosa

Induction of extrinsic and intrinsic apoptosis in cervical cancer cells by Momordica dioica mediated gold nanoparticles