NDLI: Silver nanoparticles biosynthesised using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line

Content Provider	IET Digital Library
Author	Fard, Shima Edalat Tafvizi, Farzaneh Torbati, Maryam Bikhof
Abstract	The aim of this study was to green synthesised silver nanoparticles (AgNPs) using Centella asiatica leaf extract and investigate the cytotoxic and apoptosis-inducing effects of these nanoparticles in MCF-7 breast cancer cell line. The characteristics and morphology of the green synthesised AgNPs were evaluated using transmission electron microscopy, scanning electron microscopy, UV–visible spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The MTT assay was used to investigate the anti-proliferative activity of biosynthesised nanoparticles in MCF-7 cells. Apoptosis test was performed using flow cytometry and expression of caspase 3 and 9 genes. The spherical AgNPs with an average size of 19.17 nm were synthesised. The results showed that biosynthesised AgNPs exhibited cytotoxicity, anti-cancer, apoptosis induction, and increased expression of genes encoding for caspases 3 and 9 in MCF-7 cancer cells in a concentration- and time-dependent manner. It seems that green synthesised AgNPs have potential uses for pharmaceutical industries.
Starting Page	994
Ending Page	1002
Page Count	9
ISSN	17518741
Volume Number	12
e-ISSN	1751875X
Issue Number	Issue 7, Oct (2018)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-nbt/12/7
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2018.5069
Journal	IET Nanobiotechnology
Publisher Date	2018-07-24
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Alloys Anti-cancer Anti-proliferative Activity Apoptosis Induction Biochemistry Biomedical Material Biosynthesised AgNPs Biosynthesised Nanoparticles Botany Cancer Caspase Gene Expression Cellular Biophysics Centella Asiatica Leaf Extract Cytotoxicity Enzymes Flow Cytometry Fourier Transform Spectra Fourier-transform Infrared Spectroscopy Genetics Green Synthesised AgNPs Green Synthesised Silver NanoParticle Infrared And Raman Spectra in Metals Infrared Spectra MCF-7 Breast Cancer Cell Line Method of Nanofabrication And Processing MTT Assay Nanofabrication Nanomedicine Nanoparticle Nanoparticle Morphology Nanotechnology Application in Biomedicine Nanotube And Nanostructured Material Optical Property of Metals And Metallic Alloy Pharmaceutical Industries Physical Chemistry of Biomolecular Solutions And Condensed State Physics of Subcellular Structure Scanning Electron Microscopy Semimetals Silver Compound Spherical AgNPs Structure of Solid Clusters Toxicology Transmission Electron Microscopy Ultraviolet Spectra UV–visible Spectroscopy Visible And Ultraviolet Spectra of Metals Visible Spectra X-Ray Diffraction
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology

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