NDLI: Role of catalytic protein and stabilising agents in the transformation of Ag ions to nanoparticles by Pseudomonas aeruginosa

Content Provider	IET Digital Library
Author	Ali, Jafar Hameed, Abdul Ahmed, Safia Ali, Muhammad Ishtiaq Zainab, Shama Ali, Naeem
Abstract	Biological routes of synthesising metal nanoparticles (NPs) using microbes have been gaining much attention due to their low toxicity and eco-friendly nature. Pseudomonas aeruginosa JP2 isolated from metal contaminated soil was evaluated towards extracellular synthesis of silver NPs (AgNPs). Cell-free extract (24 h) of the bacterial isolate was reacted with AgNO3 for 24 h in order to fabricate AgNPs. Preliminary observations were recorded in terms of colour change of the reaction mixture from yellow to greyish black. UV-visible spectroscopy of the reaction mixture has shown a progressive increase in optical densities that correspond to peaks near 430 nm, depicting reduction of ionic silver (Ag+) to atomic silver (Ag0) thereby synthesising NPs. X-ray diffraction spectra exhibited the 2θ values to be 38.4577° confirming the crystalline and spherical nature of NPs [9.6 − 26.7 (Ave. = 17.2 nm)]. Transmission electron microscopy finally confirmed the size of the particles varying from 5 to 60 nm. Moreover, rhamnolipids and proteins were identified as stabilising molecules for the AgNPs through Fourier transform-infrared spectroscopy. Characterisation of bacterial crude and purified protein fractions confirmed the involvement of nitrate reductase (molecular weight 66 kDa and specific activity = 3.8 U/mg) in the Synthesis of AgNPs.
Starting Page	295
Ending Page	300
Page Count	6
ISSN	17518741
Volume Number	10
e-ISSN	1751875X
Issue Number	Issue 5, Oct (2016)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-nbt/10/5
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2015.0093
Journal	IET Nanobiotechnology
Publisher Date	2016-10-01
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Atomic Silver Biochemistry Biomolecular Structure, Configuration, Conformation, And Active Sites Catalysis Catalytic Protein Enzymes Fourier Transform Infrared Spectra Fourier Transform-infrared Spectroscopy Heterogeneous Catalysis at SurFace And Other Surface Reactions Infrared Molecular Spectra Interactions with Radiations at The Biomolecular Level Ionic Silver Metal NanoParticle Method of Nanofabrication And Processing Microorganisms Molecular Biophysics Nanobiotechnology Nanoparticle Nitrate Reductase Optical Densities Physical Chemistry of Biomolecular Solutions And Condensed State Pseudomonas Aeruginosa Rhamnolipids Silver Compound Stabilising Agent Transmission Electron Microscopy Ultraviolet Spectra UV–visible Spectroscopy Visible Molecular Spectra Visible Spectra X-Ray Diffraction X-Ray Diffraction Spectra
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology

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