NDLI: Strategies for fabricating a biorecognition interface for a label free electrochemical immunosensor

Content Provider	IEEE Xplore Digital Library
Author	Sook Mei Khor Guozhen Liu Harper, J.B. Iyengar, S.G. Gooding, J.J.
Copyright Year	2010
Description	Author affiliation: AgaMatrix, Inc. 10 Manor Parkway Salem, NH 03079 USA (Iyengar, S.G.) \|\| School of Chemistry, University of New South Wales, Sydney NSW 2052, Australia (Sook Mei Khor; Guozhen Liu; Harper, J.B.; Gooding, J.J.)
Abstract	An electrochemical interface for a label-free electrochemical immuno-biosensor was prepared by modifying electrodes with a mixed layer comprising a molecular wire (MW) and oligo(ethylene glycol) (OEG) molecules derived from in situ-generated aryl diazonium salts. The in situ-generated aryl diazonium salts were prepared in two different ways using either organic solvents or aqueous solutions. For organic phase surface modification, the corresponding amines of MW and OEG were dissolved in acetonitrile while for aqueous phase surface modification the corresponding amines of MW and OEG were dissolved in 0.5 M HCl acidic solution. Reduction of the aryl diazonium cations generates the corresponding aryl radicals which react with the carbon substrate, resulting in a covalent bond. Our results show that the interface generated in acidic aqueous conditions not only produced denser layers on the electrode surfaces, as determined by the passivation of the electrode towards the redox active species such as $Fe(CN)^{3−/4−}$ and $Ru(NH^{2+/3+},$ but also has good responses for the detection of small molecules such as biotin.
Starting Page	230
Ending Page	233
File Size	557667
Page Count	4
File Format	PDF
ISBN	9781424452613
DOI	10.1109/ICONN.2010.6045168
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-02-22
Publisher Place	Australia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Fabrication label-free electrochemical immuno-biosensor oligo(ethylene glycol) molecular wire Wires Media aryl diazonium salts Biosensors Carbon Immune system
Content Type	Text
Resource Type	Article

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