NDLI: Characteristics of niosomes entrapped with rice bran bioactive compounds prepared by supercritical carbon dioxide

Content Provider	IEEE Xplore Digital Library
Author	Manosroi, A. Chutoprapat, R. Abe, M. Manosroi, J.
Copyright Year	2010
Description	Author affiliation: Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand (Manosroi, A.; Chutoprapat, R.; Manosroi, J.) \|\| Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Chiba, Japan (Abe, M.)
Abstract	Bioactive compounds [ferulic acid(F), γ-oryzanol(O) and phytic acid(P)] in rice bran are unstable antioxidants. Niosomes composed of Tween61 mixed with cholesterol at 1:1 molar ratio which gave the highest physical stability at 4, 30 and 45 °C for 3 months, were selected to entrap the semi-purified rice bran extracts containing F, O and P at 0.5, 1.5 and 1.5%w/w, respectively as well as their combination by supercritical carbon dioxide (scCO2) or chloroform film with sonication. The entrapment efficiency of F, O and P in niosomes was determined by gel filtration. The characteristics including vesicular size, morphology, dispersibility, phase transition temperature (Tc) and microviscosity of the niosomal dispersion were also investigated by dynamic light scattering (DLS) apparatus, freeze-fracture transmission electron microscope (FF-TEM), visual observation, differential scanning calorimeter (DSC) and the fluorescence polarization technique, respectively. The niosomes entrapped and not entrapped with the semi-purified rice bran extracts by both scCO2 and the chloroform film technique were stable vesicles with the size of 300–1500 nm. The morphology of all niosomes prepared by scCO2 and chloroform film technique were in large unilamellar vesicles (LUVs) and the mixture of unilamellar and multilameller vesicles (MLVs), respectively. The Tc of all niosomes prepared by both methods was 75 to 81 °C with the AH of +0.337 to +0.661 kJ/mol. The LUV niosomes by scCO2 gave higher entrapment efficiencies of the hydrophilic bioactive compounds, F (64.47±1.17%) and P (54.85±0.11%) and lower entrapment efficiencies of the hydrophobic bioactive compound, O (47.54±2.31%) than niosomes by the chloroform film which gave at 53.56±1.76 (F), 60.79±0.65 (P), 53.48±0.15 (O) %, respectively, of about 1.3 times. This study has indicated that the rice bran bioactive compounds did not only not interfere with the Tc and microviscosity of niosomes prepared by both methods, but also the niosomes prepared by scCO2 technique can enhance the entrapment efficiency of these bioactive compounds.
Starting Page	24
Ending Page	28
File Size	420407
Page Count	5
File Format	PDF
ISBN	9781424452613
DOI	10.1109/ICONN.2010.6045162
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-02-22
Publisher Place	Australia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	characteristics phytic acid Films Carbon dioxide Fluorescence rice bran extracts chloroform film method niosomes supercritical carbon dioxide fluid Temperature measurement ferulic acid γ-oryzanol Morphology Biomembranes
Content Type	Text
Resource Type	Article

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