NDLI: An electroporation microchip for gene transfection and system optimization

Content Provider	IEEE Xplore Digital Library
Author	Min Li Kuo-Chin Lin Chi-Chang Su Yu-Tsung Wang Chun-Sheng Fang Yu-Cheng Lin
Copyright Year	2005
Description	Author affiliation: Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan (Min Li; Kuo-Chin Lin; Chi-Chang Su; Yu-Tsung Wang; Chun-Sheng Fang; Yu-Cheng Lin)
Abstract	This paper describes and characterizes an electroporation microchip device and system optimization. This device combines microfabrication techniques, logic circuit, and electrophoresis design to create a multi-function gene transfection device that can be used in a number of medical science research applications. This device consists of two components: a cell-accommodation cavity and electrodes for providing electroporation and electrophoresis electric power. We demonstrated the ability to use electrophoresis force to increase the gene concentration on site-specific surface of cell lines which further enhanced the gene delivery. Meanwhile, the parameters of the electroporation system, which could have an influence on the delivery rate, were optimized by the Taguchi method, resulting in optimized values of 50 mum electrode gap, 80 mug/mL pEGFP-N1 concentration, 6 V applied transfection voltage, and 2-pulse per time with above 95 % confidence. Experimental results showed that the efficiency of gene transfection with an attracting-electric field become much higher than that without an attracting-electric field, and the delivery rate was up to 35.89 % when utilizing GFP genes into NIH-3T3 cells. The electrostatic force can be designed into specific regions, where the DNA plasmids are attracted to provide the region-targeting function. The adherent cells could be manipulated in situ without detachment by this electroporation microchip. The system has several advantages of portable, cost-effective, high transfection rate and easy operation.
Sponsorship	IEEE Ind. Electron. Soc. (IES) Chinese Inst. of Autom. Eng. (CIAE) Nat. Sci. Council, Taiwan, R.O.C. Minist. of Educ., Taiwan, R.O.C
Starting Page	794
Ending Page	798
File Size	827709
Page Count	5
File Format	PDF
ISBN	0780389980
DOI	10.1109/ICMECH.2005.1529363
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2005-07-10
Publisher Place	Taiwan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrokinetics DNA Gene therapy Optimization methods Voltage Cells (biology) Logic devices Logic design Biomedical electrodes Electrostatics
Content Type	Text
Resource Type	Article

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