NDLI: The application of an innovative MEMS protein chip in real-time total internal reflection fluorescence microscopy

Content Provider	IEEE Xplore Digital Library
Author	Yen, Y.K. Lee, J.Y. Kuo, M.C. Huang, L.S.
Copyright Year	2005
Description	Author affiliation: Inst. of Appl. Mech., Nat. Taiwan Univ., Taipei, Taiwan (Yen, Y.K.; Lee, J.Y.; Kuo, M.C.; Huang, L.S.)
Abstract	Single biomolecular detection and real-time motion tracking of an anti-IgG molecule in a microchannel was successfully demonstrated by using total internal reflection fluorescence (TIRF) microscopy. Fluorescence-labeled biomolecules were excited at the transparent near-wall region by the evanescent wave which occurred at the optically index-mismatch interface. The MEMS-based microchannels biochip was also well designed and fabricated to exploit to monitor the motion of a single biomolecule in the near-wall flow layer. The motion of a single anti-IgG molecule has been tracked and analyzed under the speed limit 6 mm/s of image capturing system. The 3D positions of a molecule were also plotted to illustrate the biomolecular trajectory.
Sponsorship	IEEE Ind. Electron. Soc. (IES) Chinese Inst. of Autom. Eng. (CIAE) Nat. Sci. Council, Taiwan, R.O.C. Minist. of Educ., Taiwan, R.O.C
Starting Page	280
Ending Page	283
File Size	699553
Page Count	4
File Format	PDF
ISBN	0780389980
DOI	10.1109/ICMECH.2005.1529269
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2005-07-10
Publisher Place	Taiwan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Micromechanical devices Proteins Fluorescence Microscopy Optical reflection Tracking Microchannel Molecular biophysics Motion detection Biomedical optical imaging
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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