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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Hsi-Kai Wang Cheng-Han Tsai Chung-Tao Tang Pi-Chun Li Jiun-Shyang Leou Yin-Liang Tang Hsyue-Jen Hsieh Han-Chung Wu Chao-Min Cheng |
| Copyright Year | 2013 |
| Description | Author affiliation: Inst. of Cellular & Organismic Biol., Acad. Sinica, Taipei, Taiwan (Chung-Tao Tang; Pi-Chun Li; Jiun-Shyang Leou; Yin-Liang Tang; Han-Chung Wu) || Dept. of Chem. Eng., Nat. Taiwan Univ., Taipei, Taiwan (Hsyue-Jen Hsieh) || Inst. of Nanoengineering & Microsyst., Nat. Tsing Hua Univ., Hsinchu, Taiwan (Hsi-Kai Wang; Cheng-Han Tsai; Chao-Min Cheng) |
| Abstract | Dengue fever is one of the acute flavivrus-borne infectious diseases caused by dengue virus with four serotypes. To date, there are no efficient diagnostic tools available for monitoring the disease activity of dengue fever. It is needed to develop a diagnostic device with the characteristics of inexpensiveness, ease-to-use and robustness for the detection of dengue fever. In this study, we have developed an ELISA-based diagnostic device prepared via wax printing method through using filter paper. The antigens that we targeted to quantify in the buffer system were both non-structural protein 1 with the detection limit of about 100 pg/mL and envelope protein with the diluted virus culture soup of about 100 times of serotype 2 dengue virus (only 2 μL sample). We believe that this study would provide insight on the development of in-vitro diagnostic devices for dengue fever and various diseases in the different divisions of medicine. |
| Starting Page | 229 |
| Ending Page | 232 |
| File Size | 535253 |
| Page Count | 4 |
| File Format | |
| e-ISBN | 9781467363525 |
| e-ISBN | 9781467363518 |
| DOI | 10.1109/NEMS.2013.6559721 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2013-04-07 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Proteins Diseases Printing Monitoring Bars Educational institutions Medical diagnostic imaging ELISA diagnostic device paper dengue |
| Content Type | Text |
| Resource Type | Article |
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