NDLI: Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy

Content Provider	IEEE Xplore Digital Library
Author	Tianbiao Zhang Changlin Zhang Zaili Dong Yifu Guan
Copyright Year	2010
Description	Author affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China (Tianbiao Zhang; Yifu Guan) \|\| State Key Laboratory of Robotics, SIA, CAS, Shenyang, China (Changlin Zhang; Zaili Dong)
Abstract	Atomic force microscope (AFM), as its high resolution and precision, was employed to directly probe intermolecular hydrogen bonds between self-assembled purines and pyrimidines. In the experiment, we directly stretch the DNA double strands that are attached to the planar modified glass surfaces and AFM tip respectively. However, distinguishing from previous literatures we focus on the NaCl density and holding time influencing the rupture force of DNA double helix and monitor the probing process under three different densities of sodium chloride (NaCl): 0mM, 50mM, and 100mM. The result shows complementary DNA strands are easier to bond as the density of NaCl increases. At the same time we test the probing process considering three holding durations: 0S, 10S, and 40S. We find the complementary DNA strands bond forming well under the situation of 40S. Then we separately do experiments with three lengths of strands: 10bp, 14bp, 20bp. Using cluster analysis method we find that the clustering center intervals are about 0.21nN, 0.29nN, and 0.39nN, and we can calculate the hydrogen bond of the single G-C is about 20pN.
Starting Page	125
Ending Page	129
File Size	115920
Page Count	5
File Format	PDF
ISBN	9781612841526
ISSN	21596972
e-ISBN	9781612841540
DOI	10.1109/NANOMED.2010.5749818
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-12-05
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Force measurement Complementary DNA strands Hydrogen bonds Force DNA Glass Artificial neural networks AFM Probes Surface treatment
Content Type	Text
Resource Type	Article

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