NDLI: Influence of chitosan on cell viability and proliferation in three dimensional collagen gels

Content Provider	IEEE Xplore Digital Library
Author	Tan, W. Krishnaraj, R. Desai, T.A.
Copyright Year	2000
Description	Author affiliation: Illinois Univ., Chicago, IL, USA (Tan, W.)
Abstract	The development of suitable three-dimensional matrices for the maintenance of cellular viability and differentiation is critical for applications in tissue engineering and cell biology. The structure and composition of the extracellular matrix (ECM) has been shown to modulate cell behavior with respect to shape, movement, proliferation and differentiation. Although collagen and chitosan have separately been proposed as in vitro ECM materials, the influence of chitosan-collagen composite matrices on cell morphology, differentiation, and function has not been examined. To this end, gel matrices of different proportions of collagen and chitosan were examined ultrastructurally and characterized in terms of tensile and shear mechanical strength and their ability to regulate cellular activity. Mechanical testing of the gels showed that matrix strength increased with chitosan proportion. Gel behavior under fluid force confirmed this result. Scanning and transmission electron microscopy indicated that the addition of chitosan greatly influences ultrastructure and changes collagen fiber crosslinking, reinforcing the structure and increasing pore size. Atomic force microscopy indicated apparent surface modifications induced by chitosan incorporation. K562 cells cultured in three-dimensional gels were examined for cell proliferation and differentiation. While cell proliferation was inhibited with an increasing proportion of chitosan, cell function based on cytokine-release was greatly augmented. Results suggest that a hybrid chitosan-collagen matrix may have potential biological and mechanical benefits for use as a cellular scaffold.
Starting Page	1509
Ending Page	1524
File Size	1297188
Page Count	16
File Format	PDF
ISBN	0780364651
ISSN	1094687X
DOI	10.1109/IEMBS.2000.898029
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2000-07-23
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrochemical machining Atomic force microscopy Cells (biology) Tissue engineering Biological cells Extracellular Shape In vitro Biological materials Composite materials
Content Type	Text
Resource Type	Article

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