NDLI: Nuclear targeting peptide scaffolds for lipofection of nondividing endothelial cells

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Volume 2 - 1999

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Proceedings of the First Joint BMES/EMBS Conference. 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Annual Fall Meeting of the Biomedical Engineering Society (Cat. No.99CH37015)

Table of contents

Intermediate filament dynamic response to shear stress in living endothelial cells

Control of endothelial focal adhesions by cell shape

Integration of total internal reflection and atomic force microscopy (TIRFM-AFM) to study stress transduction mechanisms in endothelial cells

Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress

Oscillatory equi-biaxial strain activates JNK in a frequency-dependent manner in bovine aortic endothelial cells

Shear stress increases endothelial cell-membrane fluidity

Laminar flow induces endothelial cell growth arrest through p53 phosphorylation and stabilization

Growth arrest and DNA damage inducible gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology

Arterial shear stress downregulates thrombin receptor expression and attenuates thrombin-induced response in endothelial cells

Cytoskeletal reorganization in endothelial cells subjected to shear stress and circumferential stretch simultaneously

Morphological response of endothelial cells to combined pulsatile shear stress plus cyclic stretch

3-D stress distribution in cultured endothelial cells exposed to shear stress

A model of the electrochemical equilibrium configuration of the capillary glycocalyx

A large deformation theory for the interaction of the red cell membrane with the endothelial glycocalyx

Directional secretion of human growth hormone by genetically-modified endothelial cells

mRNA expression of extracellular matrix proteins following exposure of bladder smooth muscle cells to sustained hydrostatic pressure

Active and passive biaxial mechanical properties of urinary bladder wall

Physiological cyclic stretch inhibits vascular smooth muscle proliferation

Fluid flow releases fibroblast growth factor-2 from human aortic smooth muscle cells

Extracellular signal-regulated kinase activation in mechanically stimulated adult rat cardiac fibroblasts

New algorithm for dynamic measurements of muscle sarcomere length

Development of an in vitro model of glaucoma

ICAM-1 but not E-selectin is upregulated by ionizing radiation in human umbilical vein endothelial cells and human microvascular endothelial cells

Radiation effect on the permeability and cell adhesion of the cerebral microvasculature

Theory on tunneling effect of thermal shock waves through cell membrance

Inhibition of intestinal proteases decreases cellular activation in SAO shock

Cardiovascular activating factors from the pancreas

Increased mitochondrial benzodiazepine receptors in inflamed lungs detected using multiple indicator dilution

Airway epithelial cells respond to mechanical forces applied via collagen and RGD coated magnetic beads

Can mechanically stimulated airway epithelial cells regulate fibroblast activity

The response of lung epithelial cells to transmural pressure

Alveolar epithelial injury in vitro: temporal evolution and strain rate sensitivity

The kinetics and concentration of Ti particles loading determine molecular and cellular responses of osteoblasts

Cyclic pressure affects bone cell proliferation

A novel in vitro shear device for high strain rate injury of three-dimensional co-cultures of neurons and astrocytes

Strain dependent channel activation in cultured neurons

Calcium alterations in mechanically injured neural-like cells

Differential genomic expression after in vitro mechanical injury of organotypic brain slice cultures

Investigation of the mechanism of contact guidance of neurite growth cones in magnetically-aligned collagen gels

Preliminary study on mechanism of the induction of sleep by time-varying magnetic field

The leucine-rich repeat region of GP Ib/spl alpha/ is important in the interaction of the platelet GP Ib-IX-V complex with immobilized von Willebrand factor under fluid shear stress

Neutrophil augmentation of platelet aggregation in linear shear flow

Characterizing cell motions in flowing blood

The role of GP IIb/IIIa inhibitors on the fibrinolytic resistance of platelet-fibrin thrombi

Effects of shear stress on tissue factor gene expression in endothelial cells

An in vitro model for studying drug carrier interactions with the endothelium

Membrane-cytoskeleton adhesion and detachment from cells with "excess" area

Inner complexity of molecular adhesion bonds

Surface roughness and molecular orientation strongly influence the forward but not the reverse rates of cell-bound receptor-ligand binding

Building the immunological synapse-toward a quantitative view of T cell activation

Adhesion of myocytes: cytoskeletal contributions assessed by peeling from micropatterned collagen

Visualization of neutrophil-platelet tether dynamics under flow

Selectin-mediated rolling of hematopoietic stem and progenitor cells

Endothelial cell micro-motion monitored by ECIS during monocytes application

Selectin-independent leukocyte rolling and adhesion in mice deficient in L-, E-, and P-selectin and ICAM-1

Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, /spl alpha//sub 4/ integrin and VCAM-1

Mechanico-transduction through selectins signals integrin mediated neutrophil arrest under shear flow

Development of a lateral view flow system for studies of cell deformation under flow conditions

Effect of fluid viscosity and erythrocytes on monocyte adhesion

Force modulation of selectin mediated leukocyte adhesive dynamics

Molecular mechanisms of platelet-tumor cell adhesion under flow

Cell adhesion kinetics varies with position in the cone-plate viscometer

Particle deposition in a parallel plate flow chamber

Novel rheology of the human airway smooth muscle cell

Geometric preferences of lamellipodia extension

Aspiration of human neutrophils: analysis of non-Newtonian fluid models

Contribution of intermediate filaments to cell stiffness

Implications of actin stress fiber buckling in rapidly shortened cells

Direct measurement of cell adhesiveness by atomic force microscopy

Measurement of erythrocyte membrane bilayer separation forces using fluorescence imaged magnetic tweezers

Membrane instability during erythrocyte maturation

Deformability and viscoelasticity of diabetic erythrocytes measured by microchannel flow system and atomic force microscope

Micromechanics of the red cell membrane skeleton: large, anisotropic strain and filament reorientation in a soft, deformed structure

Imaging of red blood cells based on recognition forces

Cellular mimics engineered from diblock copolymers

Effects of Ca/sup 2+/ on the temperature transition of hemoglobin properties in human red blood cells

Single molecule polarization microscopy for investigating real time structural dynamics of biological macromolecules

Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques

Reversible and irreversible unfolding of eukaryote chromosomes by force

Force dependence of E-selectin/sialyl Lewis/sup x/ single bond dissociation

Mechanical regulation of biorecognition: forced unfolding studies of the RGD-containing fibronectin type III/sub 10/ module

Intracellular fate of lipoplex plasmid DNA

Mitosis enhances transgene expression of plasmid delivered by cationic liposomes

Intramuscular co-delivery of DNA and cytokines from biodegradable microspheres alters the immune response

Nuclear targeting peptide scaffolds for lipofection of nondividing endothelial cells

Ultrasound-mediated drug delivery to prostate cancer and smooth muscle cells

Electroporation of single cells by high frequency shocks

Electroporation-mediated delivery of macromolecules to intestinal epithelia

Electroporation of prostate cancer cells for drug delivery

Diffuse double layer effects observed by dielectrophoresis of Herpes Simplex Virus, type 1 (HSV-1)

Pressure and temperature-dependence of the hydraulic conductivity in a fibrosarcoma

Controlling the orientation of immobilized proteins by genetic circular permutation

Surface modification with cell adhesion peptides alters extracellular matrix deposition

Smart bioconjugates

Prediction of antisense oligonucleotide binding affinity and activity in cell culture

Activating transcription with light using caged DNA

Dynamics of integrin-immediated signaling via FAK and ERK2

Comparison of contractile force generated by isolated and paired fibroblasts in vitro

Propulsive forces in keratocyte migration

The identification of a subgroup of leukocytes that do not respond to fluid shear stress by pseudopod retraction

Induction of cell migration on synthetic polymer substrates through tailored cell-substrate matrix reciprocity

Correlating three-dimensional cell migration to substratum adhesiveness

The differential effect of endothelial cell factors on in vitro motility of metastatic and non metastatic cells

The effect of cellular parameters on the spatial operation of an autocrine system

Mechano-transduction in endothelial cells

G protein activation in endothelial cells by stretch

Day 1 wound fluid and PDGF-BB elicit similar fibroblast behavior in vitro

An approach to ex vivo expansion of stem cells

Culture of osteoblastic cells in porous scaffolds with convection

Development of tissue engineered meniscal cartilage implants

Genetically engineered non-osteoblastic cells expressing a transcriptional activator of osteoblast differentiation

Modulation of osteoblastic phenotype by substrate-dependent changes in fibronectin conformation

Factors affecting chondrocyte phenotype during monolayer expansion

A microstructural finite element simulation of mechanically-induced bone formation

Tissue engineered neural constructs designed for intracerebral gelation

Simulated peripheral nerve regeneration in magnetically aligned collagen gels derivatized with laminin peptides and seeded with Schwann cells

Mechanical behaviour of fibroblast-populated ordered collagen-gel

3-dimensional imaging and analysis of smooth muscle colonization of porous chitosan scaffolds

Development of a new scaffold for tissue engineering

Bioreactor characterization and optimization for cartilage tissue engineering

Organ culture as a standard test for evaluation of tissue engineered arteries

A novel system for physiologic pulsatile perfusion of intact vascular tissue

Tissue-engineered microvascular networks for gene therapy

Towards a functional tissue engineered cardiac muscle

Cyclic mechanical strain stimulates in vitro remodeling of tissue engineered vascular grafts

Preliminary engineering of a biomimicking vascular graft

Three-dimensional environment promotes in vitro differentiation of cardiac myocytes

Mechanical properties of tissue engineered constructs with increased crosslinking of the extracellular matrix

Constitutive modeling of human skin allowing for anisotropy and growth

A novel system to study dermal-hepatic interactions in vitro

Effects of keratinocyte growth factor on in vitro engineered, genetically modified human epidermis: paracrine versus autocrine actions

Microfabrication of basal lamina analogs with complex topographic features

Skin adaptation: comparison of in vivo and in vitro results

A model of sarcolemmal Ca/sup 2+/ current and cytosolic Ca/sup 2+/ transient in a rat ventricular cell

Predictions for epicardial ventricular action potentials of healthy and diabetic rats

Real-time interactions between a cardiac cell and simulated cardiac cell models

Theoretical and experimental study of intercellular junction-induced sawtooth effect in cardiac cell-pairs

Optical mapping of anatomical reentry in monolayers of cultured neonatal rat cardiac myocytes

Model-augmented investigations on field-stimulated cardiomyocytes

Analysis using a modified Luo-Rudy model of ventricular action potentials

The influences of cardiac mechano-electrical feedback on action potential duration

Role of calcium-activated chloride currents in heart in pathological conditions: a computer model

A Chebyshev pseudospectral method for cardiac propagation modeling

3D conduction in the bullfrog trabeculum

Bidomain modeling of cardiac tissue on a message-passing parallel computer

Propagation from a real ventricular cell to a two-dimensional sheet of model cells

Electrotonic suppression of early after depolarizations at the Purkinje-ventricular interface

Calcium transient in cardiac cells during discontinuous conduction

Suppression of beat to beat repolarization variability by electrical coupling in guinea pig ventricular myocytes

Propagation of the cardiac action potential from an ectopic focus into a two-dimensional sheet of ventricular cells

A model study of the effects of sodium conductance changes on the inducibility of cardiac reentrant activity

Mathematical models of cardiac arrhythmias derived from experimental recordings

Modeling induction of reentry with a realistic S2 stimulus

Fluorescent imaging of the vortex core in the rabbit heart

Critical point theory versus the bidomain model for reentry induction

Existence of bistability and correlation with arrhythmogenesis in sheep atria

Low-dimensional model of reentry in loop of cardiac cells

Curvature effects on propagation in cardiac tissue

Accurate localization of phase singularities during reentry

Wave vector analysis of cardiac activation patterns

The relationship between propagation "vorticity" and the repolarization field during atrial activation

A new mechanism for cardiac spiral wave meandering

A confocal setup to measure transmembrane potential in cardiac tissue with very high spatial and temporal resolution

Field induced membrane potential changes in small regions of guinea pig papillary muscles

Seeking a model for study of arrhythmia induction by nonuniform polarization

Superelastic endocardiac multi-electrode

Development of a simple biosensor mapping technology for epicardial activation in rodent hearts

Fluid dynamics of valve closure and sustained regurgitation in cardiac valve prostheses

Effect of LV pressure on deformation in the mitral valve

Cardiac simulations of regurgitant jets in mitral valve, for different pathological cases, in steady and pulsatile flow

A three-dimensional coupled fluid-structure computational model of mitral valve function

Real time deformation of the bioprosthetic heart valve

Mechanical properties of explanted porcine bioprosthetic heart valves

Bioprosthetic heart valve leaflet deformation monitored by double pulse stereo photogrammetry

A new aortic root prosthesis with compliant sinuses for valve sparing operations

Protocols for the viscoelastic analysis of porcine aortic valve leaflets: validation through QLV theory

In vitro investigation on opening angle and hydrodynamic performance of four bileaflet valves in mitral position

Leakage flow through mechanical prostheses: an initiator of platelet damage

Alignment maps for heart valve leaflets

Flow affects the biosynthetic activity of aortic valve leaflet fibroblasts

Dynamic imaging of BHV cuspal motion using laser projection

Non-invasive determination of mitral valve acoustic properties: a proposed method to determine tissue alterations due to disease

Does myxomatous mitral valve disease selectively affect the chordae? A mechanical and biochemical analysis

The origin and consequence of "negative compliance" in bioprosthetic heart valves

Visualization and analysis of left ventricular wall motion

Robust segmentation of myocardial tissue in ultrasound images

Derivation of the diastolic left ventricular wall-thinning to transmitral flow-velocity relation: validation by model-based image processing

The analysis of mechanical remodelling as an approach to the assessment of the myocardium contractility

Measuring the performance of EKG-based image selection for the purpose of cardiac calcification scoring

Predicting ejection fraction improvement by mechanical model and cine magnetic resonance images

Time-dependent vorticity field in vascular junctions

Chronic vessel ligation increases vessel wall strain and vessel length density in mesenteric microvascular networks

Effect of different POS:DOS ratios on hemodynamics at the distal end-to-side anastomosis of a bypass graft

Biaxial mechanical properties of dilated human ascending aortic tissue

Double molecular tracer for myocardial regional flows

Correlation between paced epimyocardial electrograms and stroke volume in heart transplants

Neither high [K/sup +/]/sub e/, low pH/sub e/ nor Ca/sup +2/ manipulations significantly impacts the correlation between MF and MDE to altered reperfusion flows following IVF

Gender difference in rheological properties of blood and risk of cardiovascular diseases. Mathematical modeling and experimental study

Prevention of mechanical stretch-induced endothelial and smooth muscle cell death in experimental vein grafts

A modified cylindrical coordinate system for the geometric analysis of abdominal aortic aneurysms

Virtual prototyping of branching blood vessels

Segmentation and tracking of coronary arteries

Dynamic deformation effects on flow in coronary artery bifurcation models

Computational investigation of pulsatile flows and wall stresses in models of abdominal aortic aneurysms

Local elastic modulus of atherosclerotic lesions of rabbit thoracic aortas and its relation to histology

Waveform dependence of wall shear stresses in a human right coronary artery

Shear rate gradient in arteriolar bifurcations: theoretical and experimental

Phase angle between circumferential strain and wall shear stress affects endothelial cell biochemical production

Mechanical wall stresses on three-dimensionally reconstructed models of abdominal aortic aneurysm

Residual stress in swine iliac arteries

The effect of aortic coarctation on shear stresses and flow fields through the rabbit aortic bifurcation

Effects of acute hyperglycemia on in vivo velocity profiles of the left anterior descending coronary artery

Is fluid shear stress an indicator of total cavopulmonary connection efficiency?

The effect of celiac and renal artery outflows on near-wall velocities in the porcine iliac arteries

Biomechanical analysis of coronary arteries using a complementary energy model and designed experiments

Evaluation of CFR and FFR parameters by CFD modeling of the flow in a stenosed coronary artery

Time-dependent aspects of the flow in the carotid bifurcation: continuous DPIV and computational results

An in vivo method for measuring vessel wall motion and cyclic strain using magnetic resonance imaging

The effect of alternative diastolic pressure-volume relations on transmitral Doppler flow: comparison by model-based image processing

Accuracy of power Doppler angiography for the assessment of arterial stenoses

Comparison of the relationship between blood pressure and pulse wave transit times at different sites

Study on interval variability of arterial pulse

Synchronized measurements of blood flow velocity distributions in carotid, brachial and femoral arteries, and ECG in human during exercise

Regulation of cardiac pressure by ejection velocity

Right-to-left heart mechanical "crosstalk": the role of functional hydraulic properties of the myocardium

Combination of Millar and conductance catheter to assess equine left ventricular function

Processing pressure-volume diagrams (DPV) using virtual instrumentation

Human cardiac physiology in the phase plane: the ejection fraction to limit cycle area relation

Comparison between parameter optimization method for estimating E/sub max/ and the traditional method

Simultaneous application of multiple oscillometric methods for blood pressure measurement in finger

Portable instrument for beat-by-beat cardiovascular monitoring based on volume-compensation and bio-admittance method

A modeling solution to the origin of the electrical impedance cardiogram

Ventricular diastolic impedance Z(t): validation of a new, model-based image processing derived index of global diastolic function

Use of a multi-mechanism constitutive model for inflation of cerebral arteries

The role of nitric oxide in the cerebral vasodilatation during hypercapnia

In vitro model of rapid intravenous fluid administration

Blood volume based dialysis patient-identification

Computer aided design of right heart bypass (Fontan) operations

Monitoring development of suction in an LVAD

Response of the elastance-based control due to changes in afterload and contractility

Estimation of systolic blood pressure variations from pulse arrival time using a narrow-band filter

The Starling forces that oppose filtration in the presence of tissue loading

Effect of intraluminal thrombus on local abdominal aortic aneurysm wall strength

The impact of ejection on ventricular performance

Time-domain analysis of the significance of wave reflections on ventricular outflow

Hypertension of cardiac origin: a model study

Responses of continuous arterial volume and arterial blood pressure to Valsalva maneuver

Energy gains/losses of normal and Fontan circulations in lambs under varying respiration parameters

Numerical simulation of flow in model, total cavopulmonary connections

Simulation of the human arterial system-static and dynamic

Mathematical simulation of the cardiovascular responses to lower body negative pressure up to -40 mmHg

Modeling and identification of cardiovascular hemodynamics in a closed-loop model with ventricular interaction

Validation on an adaptive hemodynamic controller

Grey box adaptive control of mean arterial blood pressure

Multiple model predictive control of canine hemodynamic variables

Baroreceptor response to modeled aortic pressure

Transitions between 2:1 and 1:1 responses in cardiac muscle induced by added stimuli

Observer of autonomic cardiac outflow based on RR and QT intervals

A new approach used to extract fluctuations in vagal activity-Wigner distribution of heart rate variability

Time-frequency analysis of the heart-rate variability during physical and physical-pharmacological tests

Method of analyzing the relationship of electrical and mechanical events of the heart during left vagal stimulation

ECG based detection of body position changes

Quantification of real-time ECG data compression algorithms

Automated QRST subtraction algorithm for analysis of T wave obscured ectopic atrial beats

A nevv method for detecting late potentials using multichannel ECGs

A fast method for identifying the equivalent cardiac dipole from the surface ECG. II. Resolvability of two epicardial pacing sites in an experimental animal model

Exercise magnetocardiography in healthy subjects and single vessel coronary artery disease patients

Real-time detection of premature ventricular contraction using a matched filter bank

Classification of QRS morphology in Holter monitoring

Wavelet-transform-based QRS complex detection in on-line Holter systems

Frequency trends of atrial fibrillation using the surface ECG

ECG waveform classification using the neural network and wavelet transform

Lossless compression of ECG signals

Closed-chest validation of source imaging from human ECG and MCG mapping data

Reconstruction of endocardial multielectrode basket geometry from multiplane fluoroscopic images

Imposition of frequency domain constraints in inverse electrocardiography

Fusion of body surface potential and Laplacian signals for electrocardiographic imaging

Inverse problem of body surface potential mapping using self-constraint super resolution (SCSR) method

Real time endocardiac volumetry

Three-dimensional visualization of epifluorescent electrodynamics

Relationship between restitution and the breakdown of reentry into fibrillation in a 3-D model of cardiac tissue

Evaluation of approximate entropy for separation of ventricular tachycardia and fibrillation

The mechanism by which pacing rate affects the upper limit of ventricular vulnerability to fibrillation by T-wave stimulation

Changes in right ventricular geometry during fibrillation [cardioverter/defibrillator application]

Differences in cardiac activation times for endocardium and epicardium in response to external electric shock

Reduced bidomain model of defibrillation

Virtual electrodes in the termination of ventricular fibrillation in protective zones

Virtual electrodes induced throughout bulk myocardium by ICD defibrillation

Computer controlled circuitry for rapid switching between implanted electrodes during a biphasic defibrillation shock

Rapid switching between transverse electrode pairs within a biphasic shock lowers implantable defibrillator thresholds

External ventricular defibrillation with a five element cluster electrode

A compact, electrode-mounted, ECG R-R interval recording system

A new image format 12-lead electrocardiogram display method

Weighted averaging of ECG signal based on the number of turning points

Non-linear adaptive prediction based ECG signal filtering

Chronic chagasic patients classification using normalized cumulants

Performance of the Hilbert transform in fetal phonocardiography for cardiotacogram generation

A two-years trend in the recovery speed of heart rate during bathing stress

Validation of Laplacian sensor characteristics

Portable real-time body surface Laplacian ECG mapping

Towards non-invasive electrical heart imaging

Experimental study and removal of the drift of the reference potential from the unipolar electrogram

A fast method for identifying the equivalent cardiac dipole from the surface ECG. II. Resolvability of two epicardial pacing sites in an experimental animal model

A possible mechanism of abnormal Q waves in hypertrophic cardiomyopathy investigated by model and simulation studies

Analyzing cardiac mapping data, object-oriented style

Non-linear frequency modulation and phase coupling in ECG during ventricular fibrillation

Case-based learning for prediction of post-myocardial infarction outcomes

The role of ischemia and reperfusion in stretch-induced arrhythmias

Assessment of the dynamics of neurovegetative response using Wigner-Ville transform

Wavelet decomposition of heart period data

Assessment of transient change of mental workload by spectral analysis of HRV with digital filter bank

A combination approach to frequency domain analysis of short-time clinical electrocardiogram recordings

Wide-aperture microwave catheter-based cardiac ablation

Virtual RF ablation in a 3D computer model of atrial arrhythmias

Endocardiac monitoring of an ablation electrode. One step solution for inverse problem

Circumferential switching between multiple electrode pairs within a biphasic shock lowers implantable defibrillator thresholds

A novel method for analog measuring of pulse width and rise time of defibrillating shocks

A system for real-time adaptive cardiac pacing

Adaptive algorithms for heart rate reconstruction

Effects of spatio-temporal conduction anisotropy on critical interval and coherence

Effect of data segmentation and windowing on the differentiation of fibrillatory rhythms from nonfibrillatory rhythms using magnitude-squared coherence

Fitting nonlinear time-domain models of respiratory mechanics to pressure-flow data from an intubated patient

Parameter estimation and event detection in a simple model of respiratory mechanics

Time-domain analysis of lung mechanics in the ventilated patient

Potential tracking of mechanical consequences of induced emphysema and lung volume reduction in sheep

Biomechanical modeling of an alveolar sac for simulation of septal stresses in pulmonary edema

Rheological properties of lung parenchyma: relationship to structure of collagen and elastin fiber networks

Drift of ventilation can lead to unreliable response to alternating breath test in infants

Measurements of pressure between laryngoscope blade and upper airway tract during orotracheal intubation

Using a closed nose chamber for airflow measurements in a transfer impedance system

Multilayer formation during interfacial oscillation is essential for proper pulmonary surfactant function

Performance of individual groups of respiratory muscles

Classification of normal person and pulmonary function disease patients based on the respiratory sound from trachea

Noise added to mechanical ventilation improves gas exchange in acute lung injury

A computer-controlled mechanical ventilator based on a rotating vane

Estimation of respiratory dynamic mechanical properties during clinically used mechanical ventilation

Hyperpolarized gas imaging with a simple programmable gas delivery system

Automatic control of mechanical ventilation and the inspired fraction of oxygen in the premature infant: a simulation study

Sleep apnea detection using flow spectral analysis and fuzzy logic

Transtracheal jet ventilation: a life-threatening or life-saving device

Simulation of arterial oxygenation in the neonate

Computer simulation of aerosol transport and deposition in multi-generation airway models

Modeling the Fontan circulation: a computer simulation of the effects of respiration

Development and testing of an intravascular artificial lung

Alveolar volume and sequential filling impact the estimation of D/sub LCO/ and D/sub LNO/

Transport pattern in the human nasal cavity

Unsteady flows within lung bifurcations

A three-dimensional model of the human airway tree: application for airway-parenchymal interaction

Hybrid modelling and simulation of the human respiratory system

A capillary network model for pulmonary blood flow and neutrophil transit

Complexity in pulmonary arterial tree structure-function

Correlation structure of end-expiratory volume (EEV) in anesthetized rats with intact upper airway

Volume increments and crackle sounds during lung reinflation

Pulmonary surfactant: an engineering marvel

Effect of high levels of film compression on surfactant transport

Influence of bulk surfactant transport on airway reopening

The effects of breathing on surfactant transport in the lung [model for surfactant replacement therapy]

Regional gas trapping during bronchoconstriction in the prone and supine positions [using PET and radio-labeled tracers]

Scaling behavior in crackle sound during lung inflation

The physical origins of low surface tensions

Recording of patterned neural networks

Homosynaptic long-term depression of the lateral perforant pathway/dentate gyrus synapse

Stochastic resonance in simulated and in vitro hippocampal CA1 cells

Nitric oxide monitoring in hippocampal brain slices

Increased tissue nitric oxide (NO) and cortical blood flow during functional activation of rat brain

Induction of LTP is associated with increased norepinephrine levels in the dentate gyrus of the freely moving rat

Guiding PC12 cell neurite outgrowth by a homogenous NGF concentration gradient

Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine

A novel method for producing patterned biomolecule gradients

Modification of evoked responses in cultured cortical networks

Experimental results of 6 neuron VLSI circuit of lamprey unit pattern generator

Bioresorbable filaments enhance nerve regeneration

Biocompatibility of chronically implanted polymer-based intrafascicular electrodes

Recording and processing of action potentials from peripheral locust nerves

Event related potential acquisition, analysis, and training system

Selective stimulation and recording using a slanted multielectrode array

Micro-machined, polyimide stimulating electrodes with electroplated iridium oxide

Micromachined multi-channel microelectrodes with titanium nitride sites

A simultaneous multichannel recording obtained from rat cortex using a plasma etched silicon depth probe

Planar transistor array systems for extracellular recordings

Electrodeposition of iridium oxide charge injection electrodes

A multipolar precision hybrid cuff electrode for FES on large peripheral nerves

Model-based design of stimulus waveforms for selective microstimulation in the central nervous system

Finite element modeling of the neuron-electrode interface: stimulus transfer and geometry

A new dc baseline stabilization scheme for neural recording microprobes

A gate-array/multi-electrode device: circuit performance and interfacing

Prediction of a patient's response to hydroxyurea treatment using artificial neural networks

Neural network scoring of rat sleep stages

Identification by BP neural networks for signal sources produced by auditory training

Depth of anesthesia estimation by adaptive-network-based fuzzy inference system

Evaluation of nonlinear generalizations of the adaptive model theory

An oscillatory neural network scheme for temporal encoding and stimulus recognition

Force-firing rate relation of neural network model for muscle force control

A recurrent spatio-temporal neuron: advantage of potential build-up

Parameter estimation methods for neural models

A parallel nonlinear-linear neuronal model

Nonstationary stochastic resonance in a reduced-order Hodgkin-Huxley neuron

Gait generation characterized by the phase sensitive properties of synaptically coupled neurons

An ionic current model for respiratory neurons in the rat vNTS and guinea pig nucleus ambiguus

Slow going-active and passive transport in axons

Simulation of dendritic structure of pyramidal cells in the cerebral cortex

Modeling of primate optokinetic and vestibulo-ocular systems based on single unit recording of cerebellar flocculus Purkinje cell activity

A phenomenological model of auditory-nerve responses: including two-tone suppression

Evaluation of a new method for CVD estimation

Chaosmakers: rhythmicity breakers

Brain dynamics: coupling intrinsic and synaptic processes

Neuronal adaptation by varying transmission delay on recurrent excitation

An adaptive analysis of neural encoding

Classification of neural spike under nearly 0 dB signal-to-noise ratio

Neural spike classification utilizing both amplitude and temporal information

Wiener filtering and classification of neurographic recordings

A decoding filter for motor cortical (MI) neurons

Analysis of neuronal functions based on Feynman diagrams

Response of simultaneously recorded cortical neurons to perturbations in arm trajectory

Site specific variability in spinal motor output

An optimization method for analyzing noise responses of high-frequency auditory-nerve fibers

Nonlinear compression in an auditory-nerve model

Adaptive interference reduction (AIR) in cuff electrode recordings

Using multivariate logistic regression to study the relationship between mechanical inputs and neural responses in mechanoreceptor neurons

Sub-threshold motor evoked response latency estimation by TFA

Effects of sampling rate on evoked potential latency change estimation

Regularization approach to the single trial estimation of multi channel evoked potentials

Damped sinusoids in single oddball responses

Visual evoked potentials to dynamic random-dot stereograms-the influence of visual field location, disparity and interocular correlation on latency

PRBS-determined temporal frequency characteristics of VEP in glaucoma

Local transform processing of single-trial evoked potential

Independent component analysis for identification and minimization of EOG artifacts in EEG recordings

A lossless compression algorithm for multichannel EEG

Prediction of hand movement from the event-related EEG using neural networks

Neural adaptive filters for estimating brainstem auditory evoked potential

Detection of pilot state during high-G by wavelet analysis of EEG signal

Causality analysis of multichannel alpha activities using multidimensional directed information

Spatio-temporal constraints in a distributed source analysis of epileptic spikes

Comparative accuracies of EEG forward solutions

A new high-resolution EEG technique based on finite resistance network model

Forward problem solution of ESI using FEM and BEM with quadratic isoparametric elements

Preliminary study of relationship between head models and computed potentials in EEG forward computation

Median-based filtering methods for EEG seizure detection

Evolution of accumulated energy predicts seizures in mesial temporal lobe epilepsy

EEG burst detection: performance evaluation

Fractal dimension detects seizure onset in mesial temporal lobe epilepsy

The EEG Bispectral Index/sup TM/ (BIS/sup TM/): development and utility

EEG-based discrimination between Yes and No

Enhancement of EEG control signals in the development of a brain-computer interface

Training setup for control of neural prosthesis using brain-computer interface

Multi-channel analysis of human event-related cortical potentials for the development of a direct brain interface

Analysis of wavelet preprocessed auditory brainstem responses with self-organizing feature maps

Classification of movement cognitive potentials from EEG

Time-frequency and topographic representation of movement planning

A full digital video EEG system

Spatial sampling of neocortical activity by electrode grids

Chronic electrode-brain interface modeled with FEM

A PC-based data acquisition system for arrays of 100 extracellular electrodes

Quantification of sensibility to conductivity changes in bipolar rheoencephalography

The conductivity of the human skull in vivo and in vitro

The electrical control of pressure sensations: the relationship between stimulation signals and subjective intensities and areas

Intracranial pressure monitoring: application of spectral analysis to the classification of B-waves

Dynamic compression of the cerebral vasculature induced by changes of pleural pressure during mechanical ventilation

Continuous estimates of index of intracranial compliance derived from intracranial pressure recordings

Biomagnetic measurement of the neural activity in the mental image processing

Modulation of cortical magnetic field responses to speech sounds

Localizing the sources of early gamma components in auditory oddball response

Virtual depth-electrode measurement using MEG eigenspace beamformer

Applications of transcranial magnetic stimulation

Optimal coil design for magnetic stimulation of the nervous system

Estimation of focus of electric field in an inhomogeneous medium exposed by pulsed magnetic field

Electric fields induced by a toroidal coil for transcutaneous magnetic stimulation of nerves

Magnetic peripheral nerve stimulation: axial versus transverse fields

Effects of repetitive transcranial magnetic stimulation on gene expression

Use of an active microelectrode array for stimulation and recording in the central nervous system

Cortical motor prosthesis control from simultaneously recorded neurons

Modelling high pulse rate electrical stimulation: effects on inferior colliculus (ICC) units [cochlear implants application]

Integrative design and hybrid assembly of a flexible retina implant system

An epi-retinal visual prosthesis implementation

Optical signal and energy transmission for a retina implant

Cuff configuration for reduction of interfering signals in nerve cuff recordings

A theoretical analysis of epidural electrical stimulation of the spinal cord

An analysis of the effect of nodal current on the stimulating potential field in relation to myelinated fibres

Position selective stimulation of a multifascicular nerve

Endpoint forces evoked by microstimulation of the cat spinal cord

Skin contact forces derived from the human sural ENG

Comparison of different fuzzy models to extract position information from muscle afferent activity

Classification of action potentials recorded from peripheral nerves with cuff electrodes

Signal-to-noise ratio of nerve signals recorded with full and open cylinder cuff electrodes

Methods for modeling the relationship between extracellular recording variability and impedance properties of chronic neural implants

Micromachined multichannel cuff electrodes for interfacing small nerves

Analog VLSI-spinal cord interface for motor control

A middle ear piezoelectric actuator-in-vitro evaluation

Intramedullary pressure induced fluid flow in bone

The role of mechanical load-induced fluid flow in osteocyte nutrition and its clinical implications

Strain amplification in the bone mechanosensory system

Heat induced collagen denaturation and its effects on bone mechanical integrity

High resolution studies of the electromechanical properties of bone during remodeling

Development of a mechanically similar composite bone replica

Ultrasonic measurement of axial strain when tissue deforms dominantly in lateral direction-method demonstration on agar phantom

The effect of meniscus on the knee joints

A mechanical model of a growing solid tumor: implications for vascular collapse and drug transport

Correlation of collagen network integrity with the biomechanical properties of bone

Dose dependent inhibition of bone loss by low level, high frequency mechanical loading of the lower appendicular and axial skeleton of post-menopausal women

The in vivo mechanical loading history of bone

Deformable modeling of facial tissue

Serious injury in rear vehicular impacts

Head injury reduction with roll bar padding

Stereotactic analysis of spinal motion segments

Lumbar facet joint capsule model

Dynamic analysis of pediatric cervical spine

Non-radiographic analysis of the pediatric spine in adolescent idiopathic scoliosis

Air bag injuries and pediatric biomechanics

Microstructural characterization of the transition region between scar and normal tissues in a healing medial collateral ligament

Ultrasonic shear modulus reconstruction on superficial tissues-method demonstration on in vitro pork rib

Stress and strain in the human distal phalanx under indentation

Tissue retraction for model-updated image-guided neurosurgery

Rigid body simulation exploring the kinematics of a floating meniscal bearing total ankle replacement

Kinematics measurement of mobile bearing total knee arthroplasty

A method for estimating center of mass from forceplate data during quiet standing

Technique for 3-D computer animation of the TMJ

Head and neck kinematics during impact loading

Gait analysis in anterior cruciate ligament injuries

Evaluation of balance during activities of daily living

Tremor quantification using digital actigraphy

Discrepancy between the 3 dimensional joint angles and clinical, planar joint angles

A computer simulation of extremity kinematics-verification of the position and orientation of axes of motion for the arm and leg

Determination of kinematically consistent joint centers from segment orientations

Qualification of movement patterns on a continuous scale

Quantifying instability during stepping via ideal trajectory analysis

Dynamic plantar pressure in the treated clubfoot: an orthopaedic engineering study

Bio-feedback-system analysis in walking using three gyro sensors

Assessment of gait kinematics in children with myelomeningocele

Joint powers in stair climbing at different slopes

A sensory feedback control model of neuromuscular systems

Minimum jerk theory revisited

Inferring motor plan complexity using a modified principal component analysis

Controllability in humans: an exploratory study

A neurobiologically motivated generalization of the adaptive model theory of human purposive movement to the control of nonlinear systems

Control model of postural stability using LQR and fuzzy logic controller

Hysteresis cycle during arm abduction and adduction in static positions

Persistent effects of voluntary and reflex activation on the stretch reflex at the human ankle

Elbow tendon reflex in stroke and normal subjects

Ankle stiffness in response to disturbance in sagittal plane

Effects of shoulder stability on endpoint stiffness dynamics

Characteristics of proprioceptive feedback in postural control

Angular stiffness in the knee during FES and iso-velocity movement in a paraplegic

Open-loop versus closed-loop control of reflexive head movements

Relationship between muscle activation and vertebral kinematics in head-neck system of rhesus monkey during voluntary head movement

Functions of the posterior temporalis and digastric muscles during bilateral biting in the control of temporomandibular joint loading

Evidence for a relationship between abnormal static torque synergies and disturbances of planar arm movements in hemiparetic subjects: preliminary results

Postural control strategies during quiet standing in normal children

A quantitative and convenient method of evaluating tendon reflex and spasticity

Force development model of skeletal muscles, using Erlang loss system

Length dependence of the contractility of smooth muscle fibers

Development of a sarcomere model using hardware

An investigation of N-let summation mechanisms in muscle

Torque-angle relationships of human wrist muscles

Mechanical actions of individual muscles at the knee joint with varus/valgus malalignment

Moment arms of muscles at the knee during internal/external and flexion/extension motion

On the 2D dynamic conditions of human fingers: internal forces only

Biomechanic-based simulation of knee dynamics

A 3-D numerical model of the human foot structure during gait

The influence of hip joint stiffness on crutch supported paraplegic standing-a modelling study

Using SIMM2 to model muscle length changes due to negative heel rocker sole modification

Modeling shoulder function in high level tetraplegia

Model-based assessment of glenohumeral stability during functional neuromuscular stimulation

Physiological control scheme of jaw simulator JSN/2A for improving reproducibility of open-close movement

An EMG classification method based on wavelet transform [and ANN]

A /spl Pi/-/spl Sigma/ network based EMG identification method

Pattern recognition of EMG [for artificial hand project]

A pattern based continuous multifunction myoelectric control strategy

Improving myoelectric signal classification using wavelet packets and principal components analysis

Estimation of motor unit territory using surface electrode arrays

EMG onset determination using a maximum likelihood method

EMG amplitude estimation using adaptive whitening

EMG-to-torque dynamic relationship for elbow constant angle contractions

Continuous time-varying autoregressive spectrum for assessment of fatigue induced changes in SEMG signals

Classifying EMG signals using T-F representation and SVD [for artificial limb control]

Decomposition of low force, simulated EMG using wavelets

Simulation of MUAP recruitment and rate coding in frequency domain

Decomposition of electromyogram long-term recordings

Frequency dependent modelling of single motor unit surface potentials

EMG mapping-estimating dipole locations by Legendre polynomial derivatives

Blind separation of EMG and ENG cuff electrode recordings [for FES application]

Spectral analysis of bio-feedback movement using autoregressive modeling

Biomechanics of pitching with injury implications

Evaluation of muscle fatigue at several contraction levels and its application for field assessment

Evaluation of fatigue using heart rate variability and myoelectric signals during skiing

Alterations in gait dynamics in health and disease: are they independent of gait speed?

Orientation aids for the blind using ultrasonic signpost system

Reader instrument of basic texts to the teaching of blind people

Portable traveling navigation system for the blind and its application to traveling training system

Urine collection system for female wheelchair users

Neck movement patterns and functional performance for computer head controls

EOG-based glasses-type wireless mouse for the disabled

Muscle recruitment by intramuscular vs. nerve cuff electrical stimulation

A low cost, disposable, zero insertion force cable connector for trial spinal cord stimulation

A low resistance, styleted spinal cord stimulation lead and connector

Basic study of the reliable implantable FES system utilizing auricular movement

Development of the surface stimulating electrodes system for gait training

Mammalian model study of selective activation of small nerve fibres using biphasic pulses with monopolar electrode

Two point vibrotactile spatial resolution for a pulse burst stimulus: tangential versus perpendicular excitation

Electrical stimulation protocol to maintain mass and contractile force in denervated muscles

Analysis of a normal subject's standing-up for comparison with computer generated FES stimulation data

Controlling FES-assisted paraplegic standing-a case study

Neuroprosthesis for standing-up and sitting-down: experimental results

Adaptive user-specified control of movements with functional neuromuscular stimulation

Adaptation of integrated restraint (IR) technology for use in the wheelchair transportation industry

Comparison of fatigue life for three types of manual wheelchairs

Analysis of a prototype geared pushrim with a manual pushrim using physiological data

Comparison of propulsion kinetics and forearm EMG between two wheelchair pushrim designs

Evaluation of a pushrim activated electric powered wheelchair

Comparison of absorbed power to vertical acceleration when measuring whole-body vibration during wheelchair propulsion

The development of a distributed pressure measurement cushion to prevent bad sitting posture

Displacement between seating surface and test dummy during transitions with a variable configuration wheelchair

A comparison of the dynamic and static stability of power wheelchairs versus scooters

EMG activity of wrist muscles during wheelchair propulsion

Internet based system for adjusting cycle ergometer workload to moderate exercise

Variable compliance joystick for control interface research

3D visualization and modeling of the foot: precision and accuracy

In-shoe multisensory data acquisition

In vivo characterization of buttock soft tissue using quasi-linear viscoelastic modeling

Etiology of decubitus ulcers using an animal model-an analysis of blood flow inhibition and recovery phenomena around compressed area

Portable soft tissue indentor for use in clinical settings

Interface pressure and pelvic movement during wheelchair propulsion

Portable instrument for ambulatory monitoring of human posture together with walking speed-preliminary study for walking speed measurement

Artificial movement and proprioceptive function in spinal cord injured subjects

Kinematic model of wrist via marker placement during manual wheelchair propulsion

An ultra-flexible electrotactile display for the roof of the mouth

Determination of primary parameters relevant to the adequacy of haemodialysis through Taguchi method

Wrist kinematics and indicators of carpal tunnel syndrome during manual wheelchair propulsion

Control of time-critical robotic rehabilitation

Development of a second generation wearable accelerometric motion analysis system

Evaluation of an assistive controller for reaching following brain injury

Space recognition of learning disabilities

Tactile sensitivity of the tongue on photolithographically fabricated patterns

Indentation assessment of plantar foot tissue in diabetic patients

Power-assisted walking support system with imbalance compensation control for hemiplegics

Upper limb prosthesis controlled by myoelectric signal

In vivo indentation of lower extremity limb soft tissues

Gait improvement in CMT patients with orthotic devices

Effect of curvature on lower extremity residual limb models

Development of myoelectric hand end effector with voluntarily controlled compliance

A control unit for myoelectrically controlled prosthesis

Natural control of wrist movements for myoelectric prostheses

A system of interpreting somatic sensations for use with artificial hands and limbs

Lift dynamics in individuals with amputations

Nonlinear viscoelastic material property estimation for lower extremity residual limbs

A device for viscoelastic assessment of the residual limb bulk soft tissue response to load

Theoretical effectiveness of energy storage and return prosthetic devices in trans-tibial amputees

Pre-stresses due to trans-tibial socket donning: a nonlinear finite element analysis with contact

X-ray localization technique for total hip replacement operation in augmented reality for therapy (ART)

Hands free walker

A 3D visualization tool for landmark allocation

The navigation system for the visually impaired

A system assisting blind children to acquire spatial awareness through body movement

Wavelet-based feature extraction of speech signal for cochlear implants

Comparisons of speech enhancement algorithms for normal and sensorineural hearing impairment listeners

Adaptive EMG-driven communication for the disabled

Virtual images as a perceptual orthosis to assist Parkinson's disease gait disorders

Task-dependent adjustments to co-stimulation levels in functional neuromuscular stimulation systems

Control of cyclic movements as muscles fatigue using functional neuromuscular stimulation

Effects of system nonlinearities on posture adjustments using functional neuromuscular stimulation

Voluntary controlled peroneal stimulator

Design of a patient-mounted multi-sensor system for lower extremity neuroprostheses

Functional and therapeutical effects of electrical stimulation in hemiplegia

Neuro-fuzzy advisor for mouse setting in Microsoft Windows

Automatic Morse code recognition with adaptive variable-ratio threshold prediction for physically impaired persons

Evaluation of joysticks in cursor positioning task by individuals with cerebral palsy

Space perception in the propioceptive information

The sense of direction in artifical space

An EEG-based cursor control system

Wavelet-based noise reduction and compression for hearing aids

Artificial speaking device for aphasic children

Proceedings of the 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Vol.20 Biomedical Engineering Towards the Year 2000 and Beyond (Cat. No.98CH36286)

Proceedings of the 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 'Magnificent Milestones and Emerging Opportunities in Medical Engineering' (Cat. No.97CH36136)

Proceedings of 18th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Proceedings of 17th International Conference of the Engineering in Medicine and Biology Society

Proceedings of the First Regional Conference, IEEE Engineering in Medicine and Biology Society and 14th Conference of the Biomedical Engineering Society of India. An International Meet

Proceedings of 16th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Societ

1992 14th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society Volume 13: 1991

[1990] Proceedings of the Twelfth Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Images of the Twenty-First Century. Proceedings of the Annual International Engineering in Medicine and Biology Society,

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Nuclear targeting peptide scaffolds for lipofection of nondividing endothelial cells

Content Provider	IEEE Xplore Digital Library
Author	Diamond, S.L. Subramanian, A. Ranganathan, P.
Copyright Year	1999
Description	Author affiliation: Dept. of Chem. Eng., Pennsylvania Univ., Philadelphia, PA, USA (Diamond, S.L.)
Abstract	Lipofection of nondividing cells is not efficient due to poor endosome escape and nuclear import of transfected DNA. Using a T7 RNA polymerase cytoplasmic transcription assay, the authors observed that nearly 80% of confluent bovine aortic endothelium expressed lipofected plasmid, indicating that cytoplasmic localization of plasmid was widespread in a nondividing cell population, albeit inefficient since most of the lipofected DNA was observed to be in endosomes. Toward overcoming the final rate limiting step of nuclear import during nonviral gene transfer, the authors conjugated a nuclear localization signal (NLS) containing the M9 sequence of heterogeneous nuclear ribonucleoprotein (hnRNP) A1 to a cationic peptide scaffold derived from a scrambled sequence of the SV40 T-antigen consensus NLS (ScT). Lipofection of confluent endothelium with plasmid complexed with the M9-ScT conjugate resulted in 83% transfection and a 63-fold increase in marker gene expression. These levels of nonviral gene transfection are unprecedented in confluent endothelium.
File Size	142494
File Format	PDF
ISBN	0780356748
ISSN	1094687X
DOI	10.1109/IEMBS.1999.802120
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1999-10-13
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Peptides DNA RNA Polymers Bovine Sequences Gene expression Lipidomics In vitro In vivo
Content Type	Text
Resource Type	Article

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