NDLI: Rolling and adhesion of monocytes to early atherosclerotic lesions of apolipoprotein E-/-(apoE-/-) mice requires P-selectin, PSGL-1, /spl alpha//sub 4/ integrin and VCAM-1

Content Provider	IEEE Xplore Digital Library
Author	Yuqing Huo Ramos, C.L. Ley, K.
Copyright Year	1999
Description	Author affiliation: Dept. of Biomed. Eng., Univ. of Virginia Health Sci. Center, Charlottesville, VA, USA (Yuqing Huo)
Abstract	To investigate the molecular basis of mononuclear cell rolling and adhesions to vascular endothelium prone to develop atherosclerotic lesion, isolated carotid arteries from ApoE-/- and other relevant mice were perfused under physiological shear stress conditions. Carotid arteries from 10-12 weeks old ApoE-/- and C57BL/6 wide-type mice fed a Western diet for 4-5 weeks, representing early atherosclerotic lesions according to vessel histology, supported mononuclear cell (U937) attachment, rolling and adhesion, while carotid arteries from an atherosclerosis-resistant strain (BALB/C) showed no adhesion. Antibody blocking assays showed that mononuclear rolling and adhesion were significantly inhibited by treating the vessel with P-selectin antibody or treating U937 with anti-PSGL-1. Treating the vessel with VCAM-1 antibody or treating U937 with /spl alpha//sub 4//spl beta//sub 1/ integrin antibody caused rolling velocities to increase (from 86/spl plusmn/4 to 167/spl plusmn/7 /spl mu/m/s). This suggests that mononuclear cell can attach, roll and adhere on early atherosclerotic endothelium via the P-selectin-PSGL-1 and VCAM-1-/spl alpha//sub 4//spl beta//sub 1/ pathways.
File Size	134475
File Format	PDF
ISBN	0780356748
ISSN	1094687X
DOI	10.1109/IEMBS.1999.802082
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1999-10-13
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Adhesives Lesions Mice Carotid arteries Humans Stress Immune system Microscopy Biomedical engineering Capacitive sensors
Content Type	Text
Resource Type	Article

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