NDLI: Investigation of early cancerous changes in bladder tissue by autofluorescence

Content Provider	IEEE Xplore Digital Library
Author	Arendt, J.T. Levin, H.S. Klein, E.A. Manoharan, R. Feld, M.S. Cothren, R.M.
Copyright Year	1997
Description	Author affiliation: Dept. of Biomed. Eng., Cleveland Clinic Found., OH, USA (Arendt, J.T.)
Abstract	Intrinsic fluorescence (autofluorescence) and reflective spectroscopy were used to study precancerous conditions and cancer in vivo in mucosal tissue of urinary bladder. Fluorescence was induced using 370 nm and 400 nm excitation, but 400 nm gave better separation of the groups. Biopsies were taken and frozen sections imaged on a fluorescence microscope with a CCD camera. The in vivo cystoscopy study had 19 patients and the cystectomy study had 35. Peak emission was 460 nm and it decreased with increased severity of disease with a 1.6 decrease from benign mucosal tissue to CIS and 3.2 decrease to invasive cancer. Invasive cancer grouped statistically differently from benign mucosal tissue using 460 nm emission while invasive cancer and CIS grouped statistically differently from benign mucosal tissue using normalized emission at 635 nm. Dysplasia did not group statistically differently from benign mucosal tissue. The fluorophores seem to be collagen and porphyrins. In microscopic images, the predominant fluorophore seems to be collagen fluorescing in blue strands while a secondary fluorophore seems to be inflammatory cells with yellowish-green round spots.
Starting Page	2290
Ending Page	2293
File Size	352797
Page Count	4
File Format	PDF
ISBN	0780342623
ISSN	1094687X
DOI	10.1109/IEMBS.1997.758820
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1997-10-30
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Bladder Cancer Fluorescence In vivo Microscopy Computational Intelligence Society Spectroscopy Biopsy Charge coupled devices Charge-coupled image sensors
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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