NDLI: Shear stress plays a role in differentiation and migration of adventitial fibroblasts

Content Provider	IEEE Xplore Digital Library
Author	Shi, D. Mathura, R.A. Garanich, J.S. Abraham, G. Tarbell, J.M.
Copyright Year	2007
Description	Author affiliation: City Univ. of New York, New York (Shi, D.; Mathura, R.A.; Garanich, J.S.; Abraham, G.; Tarbell, J.M.)
Abstract	Rat aortic fibroblasts were cultured either subconfluent (<20%) or confluent (>80%). Western blotting was conducted to evaluate the amount of two phenotype markers of smooth muscle cells (SMCs), SM alpha-actin (alpha-SMA) and SM myosin heavy chain (SM-MHC). The results showed thatwere native fibroblasts and confluent fibroblasts were myofibroblasts. These two types of cell were then plated on slides. 8 $dyn/cm^{2}$ of shear stress was applied to fibroblasts for 6 h and myofibroblasts for 24 h, respectively. Using immunostaining we found that expressions of both alpha-SMA in fibroblasts and SM-MHC in myofibroblasts were significantly upregulated under shear by 2.3-fold and 2.2-fold, respectively, based on the increase of fluorescent intensity compared to static controls. These results indicate that shear stress can promote fibroblast differentiation into myofibroblast, and myofibroblast differentiation into SMC. In another experiment, SMCs, fibroblasts, and myofibroblasts were plated in Boyden chambers, and 13 $dyn/cm^{2}$ of shear was applied to the cells for 4 h. We found the migration of fibroblasts was enhanced by shear, whereas, the migration of both SMCs and myofibroblasts were suppressed. Taken together, we speculate that the interstitial flow shear stress might play an important role in phenotype modulation and migration of fibroblast and myofibroblast during neointima formation and vascular remodeling.
Starting Page	205
Ending Page	206
File Size	1132415
Page Count	2
File Format	PDF
ISBN	9781424410323
DOI	10.1109/NEBC.2007.4413350
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-03-10
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Stress Fibroblasts Sliding mode control Samarium Muscles Arteries Mice Biomembranes Biomedical engineering Cities and towns
Content Type	Text
Resource Type	Article

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