NDLI: A High Throughput Microfluidic Thermal Reactor

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Chen, P. -C Park, D. S. You, B. H. Kim, N. Park, T. Nikitopoulos, D. E. Soper, S. A. Murphy, M. C.
Copyright Year	2009
Abstract	A high throughput microfluidic system including a 96 continuous flow (CF) thermal reactors and a multi-zone thermal control system was designed and fabricated. An infrared camera (IR) was used to analyze and verify the uniformity of the temperature distribution. Temperature variations from the nominal values were ±2°C in the denaturation zone and ±1°C in the renaturation and extension zones. Six different DNA fragments, with lengths ranging from 99 bp to 997 bp, were obtained from a λ-DNA template, each with a distinct renaturation temperature. As an initial demonstration of the biochemical performance of the polymerase chain reaction (PCR) reactor arrays, a column device comprised of eight 25-cycle CFPCRs was used to amplify identical PCR cocktails for each DNA fragment simultaneously at a flow velocity of 2 mm/s. All but the 997 bp were successfully amplified. Yields for the 99 bp fragment varied from 15%–36% of the amplicon on block thermal cycler. In a second experiment, a row device composed of six 25-cycle CFPCRs was used to successfully, simultaneously amplify cocktails for all six DNA fragment lengths at a flow velocity of 1 mm/s in parallel. Each device in the row had a distinct renaturation temperature to match the DNA fragment length. The parallel thermal arrays can be used in modular systems including both PCR for amplification and, for example, ligase detection reaction (LDR) for mutation detection.
Starting Page	273
Ending Page	275
Page Count	3
File Format	PDF
ISBN	9780791843758
DOI	10.1115/IMECE2009-13130
e-ISBN	9780791838631
Volume Number	Volume 2: Biomedical and Biotechnology Engineering
Conference Proceedings	ASME 2009 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2009-11-13
Publisher Place	Lake Buena Vista, Florida, USA
Access Restriction	Subscribed
Subject Keyword	Cycles Chain Temperature distribution Temperature Thermal reactors Dna Control systems Flow (dynamics) Microfluidics
Content Type	Text
Resource Type	Article

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