NDLI: Investigation of Promotion of Bone Matrices in Cultured Osteoblasts by Mechanical Vibration

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Shiraishi, Toshihiko Shikata, Tetsuo Morishita, Shin Takeuchi, Ryohei
Copyright Year	2009
Abstract	This paper describes effects of mechanical vibration on osteoblasts. Their bone mass generation was investigated when sinusoidal inertia force was applied to the cells. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibration group of the culture plate was set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation of 0.5 G and 25 Hz for 24 hours a day in another incubator separated from non-vibration group during 28 days of culture. Gene expression of alkaline phosphatase (ALP) was measured by a real-time reverse transcription polymerase chain reaction method. ALP activity was measured by Azo-dye method. Calcium salts generated by the cells were observed by being stained with alizarin red S solution. As a result, it is found that the mechanical vibration accelerates the gene expression and protein generation of ALP, and the calcium salt generation.
Starting Page	465
Ending Page	466
Page Count	2
File Format	PDF
ISBN	9780791843758
DOI	10.1115/IMECE2009-11131
e-ISBN	9780791838631
Volume Number	Volume 2: Biomedical and Biotechnology Engineering
Conference Proceedings	ASME 2009 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2009-11-13
Publisher Place	Lake Buena Vista, Florida, USA
Access Restriction	Subscribed
Subject Keyword	Proteins Aluminum plate Chain Vibration Inertia (mechanics) Carbon dioxide Plates (structures) Bone Excitation Osteoblasts Excitation systems
Content Type	Text
Resource Type	Article

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