NDLI: Use of the Freely-Swimming, Serratia Marcescens Bacteria to Enhance Mixing in Microfluidic Systems

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Peysepar, Mahyar Shafii, Mohammad Behshad Rasoulian, Ramin Jamalifar, Hosein Fazeli, Mohammad Reza
Copyright Year	2009
Abstract	Mixing has become a challenge in micro-fluidic systems because of the low Reynolds number in micro-channels. The method which is implemented in this paper is to use freely-swimming bacteria to enhance the mixing process. Accordingly, the Serratia marcescens bacteria were used for this matter. The mixing performance of the system is quantified by measuring the diffusion rate of Rhodamine B in a particular section of a channel connected to a chamber with varying Rhodamine B concentration. The concentration of Rhodamine B was measured using the Laser Induced Fluorescence (LIF) technique. The channel is in the form of a pipe and is closed on the extending side. In this paper, it is demonstrated that the corresponding diffusion coefficient can be augmented by bacterial participation and that this augmentation can be continued for several hours, depending on the environmental conditions. Additionally, it is shown that the mixing process reacts in response to modifications to the chemical environment of the system, which in turn affect the metabolic activity of the bacteria. Also, a 30 mM glucose buffer was used to show the impact of food on the performance of the bacterial system. It is thus shown that the existence of glucose increases the mixing ability of bacteria.
Starting Page	463
Ending Page	467
Page Count	5
File Format	PDF
ISBN	9780791843857
DOI	10.1115/IMECE2009-11469
e-ISBN	9780791838631
Volume Number	Volume 12: Micro and Nano Systems, Parts A and B
Conference Proceedings	ASME 2009 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2009-11-13
Publisher Place	Lake Buena Vista, Florida, USA
Access Restriction	Subscribed
Subject Keyword	Microfluidic systems Bacteria Mixing Image processing Serratia marcescens Microfluidics
Content Type	Text
Resource Type	Article

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