NDLI: IFN-ß expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling.

Please wait, while we are loading the content...

The IL-2 diphtheria toxin fusion protein denileukin diftitox modulates the onset of diabetes in female nonobese diabetic animals in a time-dependent manner and breaks tolerance in male nonobese diabetic animals.

Redundant and nonredundant functions of ATM and H2AX in ß T-lineage lymphocytes.

B7-CD28 costimulatory signals control the survival and proliferation of murine and human Î³Î´ T cells via IL-2 production.

Cutting edge: Clec9A+ dendritic cells mediate the development of experimental cerebral malaria.

Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.

Helminth infection impairs autophagy-mediated killing of bacterial enteropathogens by macrophages.

Substrate rigidity regulates human T cell activation and proliferation.

Species selectivity in poxviral complement regulators is dictated by the charge reversal in the central complement control protein modules.

µ-chain-deficient mice possess B-1 cells and produce IgG and IgE, but not IgA, following systemic sensitization and inhalational challenge in a fungal asthma model.

Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice.

Critical role for CCAAT/enhancer-binding protein ß in immune complex-induced acute lung injury.

Programming of regulatory T cells from pluripotent stem cells and prevention of autoimmunity.

Virologically suppressed HIV patients show activation of NK cells and persistent innate immune activation.

Human bocavirus NP1 inhibits IFN-ß production by blocking association of IFN regulatory factor 3 with IFNB promoter.

Vaccines expressing the innate immune modulator EAT-2 elicit potent effector memory T lymphocyte responses despite pre-existing vaccine immunity.

Cathepsin B controls the persistence of memory CD8+ T lymphocytes.

Multieffector-functional immune responses of HMBPP-specific VÎ³2VÎ´2 T cells in nonhuman primates inoculated with Listeria monocytogenes ΔactA prfA*.

Vaccination with cancer- and HIV infection-associated endogenous retrotransposable elements is safe and immunogenic.

Retinal laser burn-induced neuropathy leads to substance P-dependent loss of ocular immune privilege.

Transcriptional regulator Id2 is required for the CD4 T cell immune response in the development of experimental autoimmune encephalomyelitis.

Extracellularly delivered single-stranded viral RNA causes neurodegeneration dependent on TLR7.

Cell-cell interaction with APC, not IL-23, is required for naive CD4 cells to acquire pathogenicity during Th17 lineage commitment.

Diet-induced obesity alters dendritic cell function in the presence and absence of tumor growth.

Cutting edge: in the absence of TGF-ß signaling in T cells, fewer CD103+ regulatory T cells develop, but exuberant IFN-Î³ production renders mice more susceptible to helminth infection.

The peptide-receptive transition state of MHC class I molecules: insight from structure and molecular dynamics.

Cutting edge: cell-extrinsic immune regulation by CTLA-4 expressed on conventional T cells.

Chronic activation of the kinase IKKß impairs T cell function and survival.

Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.

A protective role for human IL-10-expressing CD4+ T cells in colitis.

Chronic alcohol consumption impairs distribution and compromises circulation of B cells in B16BL6 melanoma-bearing mice.

Transcription factor zinc finger and BTB domain 1 is essential for lymphocyte development.

The adjuvant LT-K63 can restore delayed maturation of follicular dendritic cells and poor persistence of both protein- and polysaccharide-specific antibody-secreting cells in neonatal mice.

A regulatory role for IL-10 receptor signaling in development and B cell help of T follicular helper cells in mice.

Noncanonical Notch signaling modulates cytokine responses of dendritic cells to inflammatory stimuli.

Mesenchymal stem/stromal cells induce the generation of novel IL-10-dependent regulatory dendritic cells by SOCS3 activation.

Cutting edge: CTLA-4 on effector T cells inhibits in trans.

Extracellular ATP exerts opposite effects on activated and regulatory CD4+ T cells via purinergic P2 receptor activation.

Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8 ß+ intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis.

Transgenic overexpression of G5PR that is normally augmented in centrocytes impairs the enrichment of high-affinity antigen-specific B cells, increases peritoneal B-1a cells, and induces autoimmunity in aged female mice.

Mutation at positively selected positions in the binding site for HLA-C shows that KIR2DL1 is a more refined but less adaptable NK cell receptor than KIR2DL3.

Year: 2012 Volume: 189 Issue: 2

Year: 2012 Volume: 189 Issue: 1

Year: 2012 Volume: 188

Year: 2011 Volume: 187

Year: 2011 Volume: 186

Year: 2010 Volume: 185

Year: 2010 Volume: 184

Year: 2009 Volume: 183

Year: 2009 Volume: 182

Year: 2008 Volume: 181

IFN-ß expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cassatella, Marco A. Grisendi, Giulia De Gironcoli, Marzia Bussmeyer, Uta Calzetti, Federica Masala, Caterina Musso, Tiziana Le Moigne, Vincent Scutera, Sara Costantini, Claudio Bazzoni, Flavia Tamassia, Nicola
Description	Country affiliation: Italy Author Affiliation: Tamassia N ( Section of General Pathology, Department of Pathology and Diagnostics, University of Verona, 37134 Verona, Italy.)
Abstract	Upon LPS binding, TLR4 activates a MyD88-dependent pathway leading to the transcriptional activation of proinflammatory genes, as well as a MyD88-independent/TRIF-dependent pathway, responsible for the transcriptional induction of IFN-ß. Previous findings delineated that human neutrophils are unable to induce the transcription of IFN-ß in response to TLR4 stimulation. Because neutrophils do not express protein kinase C Îµ, a molecule recently reported as essential for initiating the MyD88-independent/TRIF-dependent pathway, we optimized an electroporation method to transfect PKCÎµ into neutrophils with very high efficiency. By doing so, a significant IFN-ß mRNA expression was induced, in the absence of LPS stimulation, not only in PKCÎµ-overexpressing neutrophils but also in cells transfected with a series of empty DNA plasmids; however, LPS further upregulated the IFN-ß transcript levels in plasmid-transfected neutrophils, regardless of PKCÎµ overexpression. Phosphoimmunoblotting studies, as well as chromatin immunoprecipitation assays targeting the IFN-ß promoter, revealed that IFN-ß mRNA induction occurred through the cooperative action of IRF3, activated by transfected DNA, and NF-κB, activated by LPS. Additional immunoblotting and coimmunoprecipitation studies revealed that neutrophils constitutively express various cytosolic DNA sensors, including IFN-inducible protein 16, leucine-rich repeat (in Flightless I) interacting protein-1, and DDX41, as well as that IFN-inducible protein 16 is the intracellular receptor recognizing transfected DNA. Consistently, infection of neutrophils with intracellular pathogens, such as Bartonella henselae, Listeria monocytogenes, Legionella pneumophila, or adenovirus type 5, promoted a marked induction of IFN-ß mRNA expression. Taken together, these data raise questions about the role of PKCÎµ in driving the MyD88-independent/TRIF-dependent response and indicate that human neutrophils are able to recognize and respond to microbial cytosolic DNA.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	3
Volume Number	189
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2012-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Biosynthesis Interferon-beta Neutrophils Immunology Plasmids Genetics Signal Transduction Toll-like Receptor 4 Physiology Transcriptional Activation Up-regulation Adenoviruses, Human Bartonella Henselae Cells, Cultured Cytosol Hek293 Cells Legionella Pneumophila Listeria Monocytogenes Metabolism Microbiology Rna, Messenger Transfection Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Directly transfected langerin+ dermal dendritic cells potentiate CD8+ T cell responses following intradermal plasmid DNA immunization.

A Large-Scale Sequencing-Based Survey of Plasmids in Listeria monocytogenes Reveals Global Dissemination of Plasmids

DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens

Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.

Toll-Like Receptor 4 Is Not Involved in Host Defense against Pulmonary Legionella pneumophila Infection in a Mouse Model

TLR2 and RIP2 Pathways Mediate Autophagy of Listeria monocytogenes via Extracellular Signal-regulated Kinase (ERK) Activation

The TRPM2 Ion Channel Regulates Inflammatory Functions of Neutrophils During Listeria monocytogenes Infection

Cutting edge: pulmonary Legionella pneumophila is controlled by plasmacytoid dendritic cells but not type I IFN.

Stimulants of Toll-like receptor (TLR)-2 and TLR-4 are abundant in certain minimally-processed vegetables.

IFN-ß expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling.

Similar Documents

Directly transfected langerin+ dermal dendritic cells potentiate CD8+ T cell responses following intradermal plasmid DNA immunization.

A Large-Scale Sequencing-Based Survey of Plasmids in Listeria monocytogenes Reveals Global Dissemination of Plasmids

DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens

Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.

Toll-Like Receptor 4 Is Not Involved in Host Defense against Pulmonary Legionella pneumophila Infection in a Mouse Model

TLR2 and RIP2 Pathways Mediate Autophagy of Listeria monocytogenes via Extracellular Signal-regulated Kinase (ERK) Activation

The TRPM2 Ion Channel Regulates Inflammatory Functions of Neutrophils During Listeria monocytogenes Infection

Cutting edge: pulmonary Legionella pneumophila is controlled by plasmacytoid dendritic cells but not type I IFN.

Stimulants of Toll-like receptor (TLR)-2 and TLR-4 are abundant in certain minimally-processed vegetables.

IFN-ß expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling.