| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Sugimoto, Chie Fukuyo, Yayoi Mori, Kazuyasu Roy, Chad J. Didier, Elizabeth S. Breed, Matthew W. Saito, Yohei Chiu, Kevin B. Kuroda, Marcelo J. Lackner, Andrew A. Cai, Yanhui Kim, Woong-Ki Hasegawa, Atsuhiko |
| Description |
Author Affiliation: Sugimoto C ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Hasegawa A ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Saito Y ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Fukuyo Y ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Chiu KB ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Cai Y ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Breed MW ( Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Mori K ( AIDS Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan); Roy CJ ( Division of Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Lackner AA ( Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Kim WK ( Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA 23507.); Didier ES ( Division of Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433); Kuroda MJ ( Division of Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433) |
| Abstract |
Monocyte and dendritic cell (DC) development was evaluated using in vivo BrdU pulse-chase analyses in rhesus macaques, and phenotype analyses of these cells in blood also were assessed by immunostaining and flow cytometry for comparisons among rhesus, cynomolgus, and pigtail macaques, as well as African green monkeys and humans. The nonhuman primate species and humans have three subsets of monocytes, CD14(+)CD16(-), CD14(+)CD16(+), and CD14(-)CD16(+) cells, which correspond to classical, intermediate, and nonclassical monocytes, respectively. In addition, there exist presently two subsets of DC, BDCA-1(+) myeloid DC and CD123(+) plasmacytoid DC, that were first confirmed in rhesus macaque blood. Following BrdU inoculation, labeled cells first appeared in CD14(+)CD16(-) monocytes, then in CD14(+)CD16(+) cells, and finally in CD14(-)CD16(+) cells, thus defining different stages of monocyte maturation. A fraction of the classical CD14(+)CD16(-) monocytes gradually expressed CD16(+) to become CD16(+)CD14(+) cells and subsequently matured into the nonclassical CD14(-)CD16(+) cell subset. The differentiation kinetics of BDCA-1(+) myeloid DC and CD123(+) plasmacytoid DC were distinct from the monocyte subsets, indicating differences in their myeloid cell origins. Results from studies utilizing nonhuman primates provide valuable information about the turnover, kinetics, and maturation of the different subsets of monocytes and DC using approaches that cannot readily be performed in humans and support further analyses to continue examining the unique myeloid cell origins that may be applied to address disease pathogenesis mechanisms and intervention strategies in humans. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| DOI |
10.4049/jimmunol.1500522 |
| Journal |
The Journal of Immunology |
| Issue Number |
4 |
| Volume Number |
195 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2015-08-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Cell Differentiation Dendritic Cells Cytology Metabolism Monocytes Animals Antigens, Cd Antigens, Cd11c Bone Marrow Cells Immunophenotyping Macaca Myeloid Cells Phenotype Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
