| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Cantisani, Rocco Soldaini, Elisabetta Laera, Donatello Finco, Oretta Sammicheli, Chiara Nuti, Sandra Tavarini, Simona De Gregorio, Ennio Bagnoli, Fabio Rappuoli, Rino Bertholet, Sylvie Mancini, Francesca Monaci, Elisabetta Salvatore, Giulia Lofano, Giuseppe Carrisi, Corrado Rossi Paccani, Silvia |
| Description |
Country affiliation: Italy Author Affiliation: Lofano G ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Mancini F ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Salvatore G ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Cantisani R ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Monaci E ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Carrisi C ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Tavarini S ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Sammicheli C ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Rossi Paccani S ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Soldaini E ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Laera D ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Finco O ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Nuti S ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Rappuoli R ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); De Gregorio E ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Bagnoli F ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy); Bertholet S ( Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy) |
| Abstract |
Induction of persistent protective immune responses is a key attribute of a successful vaccine formulation. MF59 adjuvant, an oil-in-water emulsion used in human vaccines, is known to induce persistent high-affinity functional Ab titers and memory B cells, but how it really shapes the Ag-specific B cell compartment is poorly documented. In this study, we characterized the Ab- and Ag-specific B cell compartment in wild-type mice immunized with HlaH35L, a Staphylococcus aureus Ag known to induce measurable functional Ab responses, formulated with MF59 or aluminum salts, focusing on germinal centers (GC) in secondary lymphoid organs. Taking advantage of single-cell flow cytometry analyses, HlaH35L-specific B cells were characterized for the expression of CD38 and GL-7, markers of memory and GC, respectively, and for CD80 and CD73 activation markers. We demonstrated that immunization with MF59-, but not aluminum salt-adjuvanted HlaH35L, induced expanded Ag-specific CD73(+)CD80(-) GC B cells in proximal- and distal-draining lymph nodes, and promoted the persistence of GC B cells, detected up to 4 mo after immunization. In addition to increasing GC B cells, MF59-adjuvanted HlaH35L also increased the frequency of T follicular helper cells. This work extends previous knowledge regarding adaptive immune responses to MF59-adjuvanted vaccines, and, to our knowledge, for the first time an adjuvant used in human licensed products is shown to promote strong and persistent Ag-specific GC responses that might benefit the rational design of new vaccination strategies. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| Journal |
The Journal of Immunology |
| Issue Number |
4 |
| Volume Number |
195 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2015-08-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
B-lymphocytes Cytology Immunology Cell Differentiation Germinal Center Polysorbates Squalene Vaccination Adjuvants, Immunologic Animals Antibodies, Bacterial Antibody Specificity Antigens, Cd Metabolism Bacterial Toxins Chemotaxis, Leukocyte Hemolysin Proteins Immunophenotyping Lymph Nodes Lymphocyte Activation Mice Phenotype Staphylococcal Vaccines Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
