NDLI: High-speed microscopy with an electrically tunable lens to image the dynamics of in vivo molecular complexes.

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High-speed microscopy with an electrically tunable lens to image the dynamics of in vivo molecular complexes.

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High-speed microscopy with an electrically tunable lens to image the dynamics of in vivo molecular complexes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hiroi, Noriko Shrestha, Roshan Taniguchi, Atsushi Funahashi, Akira Tamura, Naoka Nakai, Yuichiro Nonaka, Shigenori Boilot, Viviane Ozeki, Mitsunori Oku, Hiromasa Clark, Joanna Draviam, Viji M. Hiraiwa, Takumi Tanimoto, Ryuichi
Description	Country affiliation: Japan Author Affiliation: Nakai Y ( Department of Biosciences and Informatics, Keio University, Hiyoshi Kohoku Ward, Yokohama, Japan.); Ozeki M ( Department of Biosciences and Informatics, Keio University, Hiyoshi Kohoku Ward, Yokohama, Japan.); Hiraiwa T ( Department of Biosciences and Informatics, Keio University, Hiyoshi Kohoku Ward, Yokohama, Japan.); Tanimoto R ( Department of Biosciences and Informatics, Keio University, Hiyoshi Kohoku Ward, Yokohama, Japan.); Funahashi A ( Department of Biosciences and Informatics, Keio University, Hiyoshi Kohoku Ward, Yokohama, Japan.); Hiroi N ( Department of Biosciences and Informatics, Keio University, Hiyoshi Kohoku Ward, Yokohama, Japan.); Taniguchi A ( Imaging Science, National Institute for Basic Biology, Okazaki, Japan.); Nonaka S ( Imaging Science, National Institute for Basic Biology, Okazaki, Japan.); Boilot V ( Department of Genetics, University of Cambridge, Cambridge, United Kingdom.); Shrestha R ( Department of Genetics, University of Cambridge, Cambridge, United Kingdom.); Clark J ( Department of Genetics, University of Cambridge, Cambridge, United Kingdom.); Tamura N ( Department of Genetics, University of Cambridge, Cambridge, United Kingdom.); Draviam VM ( Department of Genetics, University of Cambridge, Cambridge, United Kingdom.); Oku H ( Division of Electronics and Informatics, Gunma University, Kiryu, Japan.)
Abstract	We provide an evaluation for an electrically tunable lens (ETL), combined with a microscope system, from the viewpoint of tracking intracellular protein complexes. We measured the correlation between the quantitative axial focus shift and the control current for ETL, and determined the stabilization time for refocusing to evaluate the electrical focusing behaviour of our system. We also confirmed that the change of relative magnification by the lens and associated resolution does not influence the ability to find intracellular targets. By applying the ETL system to observe intracellular structures and protein complexes, we confirmed that this system can obtain 10 nm order z-stacks, within video rate, while maintaining the quality of images and that this system has sufficient optical performance to detect the molecules.
ISSN	00346748
Issue Number	1
Volume Number	86
e-ISSN	10897623
Journal	Review of Scientific Instruments
Language	English
Publisher	American Institute of Physics
Publisher Date	2015-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electrical Equipment And Supplies Intracellular Space Metabolism Lenses Microscopy, Fluorescence Instrumentation Methods Proteins Computer Simulation Green Fluorescent Proteins Hela Cells Humans Imaging, Three-dimensional Microtubules Pattern Recognition, Automated Video Recording Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Physics Discipline Chemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Instrumentation

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