NDLI: Inclined selective plane illumination microscopy adaptor for conventional microscopes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cutrale, Francesco Gratton, Enrico
Description	Country affiliation: United States Author Affiliation: Cutrale F ( Laboratory for Fluorescence Dynamics, Biomedical Engineering Department, University of California, Irvine, California, USA.)
Abstract	Driven by the biological sciences, there is an increased need for imaging modalities capable of live cell imaging with high spatial and temporal resolution. To achieve this goal in a comprehensive manner, three-dimensional acquisitions are necessary. Ideal features of a modern microscope system should include high imaging speed, high contrast ratio, low photo-bleaching and photo-toxicity, good resolution in a 3D context, and mosaic acquisition for large samples. Given the importance of collecting data in live sample further increases the technical challenges required to solve these issues. This work presents a practical version of a microscopy method, Selective Plane Illumination Microscopy re-introduced by Huisken et al. (Science2004,305,1007-1009). This method is gaining importance in the biomedical field, but its use is limited by difficulties associated with unconventional microscope design which employs two objectives and a particular kind of sample preparation needed to insert the sample between the objectives. Based on the selective plane illumination principle but with a design similar to the Total Internal Reflection Fluorescence microscope, Dunsby (Dunsby, Opt Express 2008,16,20306-20316) demonstrated the oblique plane microscope (OPM) using a single objective which uses conventional sample preparation protocols. However, the Dunsby instrument was not intended to be part of a commercial microscope. In this work, we describe a system with the advantages of OPM and that can be used as an adaptor to commonly used microscopes, such as IX-71 Olympus, simplifying the construction of the OPM and increasing performance of a conventional microscope. We named our design inclined selective plane illumination microscope (iSPIM).
File Format	HTM / HTML
ISSN	1059910X
e-ISSN	10970029
DOI	10.1002/jemt.22089
Journal	Microscopy Research and Technique
Issue Number	11
Volume Number	75
Language	English
Publisher	Wiley-Liss
Publisher Date	2012-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Chemistry Techniques Imaging, Three-dimensional Microscopy, Fluorescence Animals Cell Line, Tumor Green Fluorescent Proteins Genetics Metabolism Mice Staining And Labeling Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Anatomy Histology Instrumentation Medical Laboratory Technology

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