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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ilmer, Mathias Liesche, Friederike Kölbl, Alexandra C. Jeschke, Udo Hiller, Roman A. Andergassen, Ulrich Hutter, Stefan Heublein, Sabine Schröder, Lennard Friese, Klaus |
| Description | Country affiliation: Germany Author Affiliation: Kölbl AC ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Hiller RA ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Ilmer M ( Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA.); Liesche F ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Heublein S ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Schröder L ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Hutter S ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Friese K ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Jeschke U ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.); Andergassen U ( Department of Obstetrics and Gynecology, LudwigMaximiliansUniversity of Munich, Munich D80337, Germany.) |
| Abstract | Altered glycosylation is a predominant feature of tumour cells; it serves for cell adhesion and detachment, respectively, and facilitates the immune escape of these cells. Therefore changes in the expression of glycosyltransferase genes could help to identify circulating tumour cells (CTCs) in the blood samples of cancer patients using a quantitative polymerase chain reaction (PCR) approach. Blood samples of healthy donors were inoculated with certain numbers of established breast cancer cell line cells, thus creating a model system. These samples were analysed by quantitative PCR for the expression of six different glycosyltransferase genes. The three genes with the best results in the model system were consecutively applied to samples from adjuvant breast cancer patients and of healthy donors. FUT3 and GALNT6 showed the highest increase in relative expression, while GALNT6 and ST3GAL3 were the first to reach statistically significant different ∆CT-values comparing the sample with and without addition of tumour cells. These three genes were applied to patient samples, but did not show any significant results that may suggest the presence of CTCs in the blood. Although the relative expression of some of the glycosyltransferase genes exhibited reasonable results in the model system, their application to breast cancer patient samples will have to be further improved, e.g. by co-analysis of patient blood samples by gold-standard methods. |
| ISSN | 17912997 |
| e-ISSN | 17913004 |
| Journal | Molecular Medicine Reports |
| Issue Number | 2 |
| Volume Number | 12 |
| Language | English |
| Publisher | Spandidos Publications |
| Publisher Date | 2015-08-01 |
| Publisher Place | Greece |
| Access Restriction | Open |
| Subject Keyword | Tumor Markers, Biological Genetics Breast Neoplasms Diagnosis Glycosyltransferases Neoplastic Cells, Circulating Metabolism Blood Pathology Cell Line, Tumor Fucosyltransferases N-acetylgalactosaminyltransferases Isolation & Purification Real-time Polymerase Chain Reaction Sialyltransferases Up-regulation Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
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