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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Yuan-Liang Luo, Guang-Heng Chen, Zong-Ping Liang, Guo-Biao Chen, An-Jian Zhuang, Yong-Xiang Wang, Xin Lu, Yi-Ping Bao, Ding-Su Guo, Ya-Nan Li, You-Ping |
| Description | Author Affiliation: Liang GB ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Luo GH ( Department of Urology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550005, P.R. China.); Bao DS ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Chen AJ ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Zhuang YX ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Guo YN ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Wang X ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Wang YL ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Chen ZP ( Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.); Lu YP ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Li YP ( Transplantation Immunology Laboratory, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.) |
| Abstract | Chronic allograft nephropathy (CAN) is a major cause of graft loss following kidney transplantation and may result from the interactions of various immune and non-immune factors. The aim of the present study was to establish an in vitro model of glomerular mesangial cell injury in order to examine the gene expression levels of indoleamine 2,3-dioxygenase (IDO), heme oxygenase-1 (HO-1) and interleukin-7 (IL-7) in mesangial cells during the healing process as well as to investigate the effects of various immunosuppressants on the expression of these genes. The HBZY-1 glomerular mesangial cell line was pre-treated in vitro with cytochalasin B for 2 h to induce reversible damage. Following the pre-treatment, the HBZY-1 cells were divided into five groups: Blank control group, cyclosporine A (CsA) group, tacrolimus (Tac) group, mycophenolate mofetil (MMF) group and rapamycin (RAPA) group. After treating the mesangial cells with each immunosuppressive drug for 6, 12 or 24 h, the mRNA and protein expression levels of IDO, HO-1 and IL-7 were examined using reverse transcription quantitative polymerase chain reaction (RT-qPCR), western blot and immunohistochemical analyses. The results showed that expression levels of HO-1 were significantly upregulated in response to treatment with CsA, FK506, RAPA and MMF, whereas the expression levels of IL-7 were markedly downregulated by treatment with the above immunosuppressants. CsA, FK506 and MMF significantly enhanced the expression levels of IDO, whereas RAPA exhibited no apparent effect on IDO. The present study may contribute to the understanding of the pathogenesis of CAN and provide novel strategies for the prevention and treatment of CAN. |
| ISSN | 17912997 |
| e-ISSN | 17913004 |
| DOI | 10.3892/mmr.2015.3713 |
| Journal | Molecular Medicine Reports |
| Issue Number | 2 |
| Volume Number | 12 |
| Language | English |
| Publisher | Spandidos Publications |
| Publisher Date | 2015-08-01 |
| Publisher Place | Greece |
| Access Restriction | Open |
| Subject Keyword | Glomerular Mesangium Drug Effects Heme Oxygenase-1 Genetics Immunosuppressive Agents Pharmacology Indoleamine-pyrrole 2,3,-dioxygenase Interleukin-7 Cell Line Cyclosporine Cytochalasin B Gene Expression Regulation Cytology Metabolism Antagonists & Inhibitors Mycophenolic Acid Analogs & Derivatives Rna, Messenger Signal Transduction Sirolimus Tacrolimus Discipline Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
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