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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhu, Jinxiang Wu, Kai Ma, Longan |
| Description | Author Affiliation: Wu K ( Department of General Surgery, The Third Affiliated Hospital (Shaanxi Provincial People's Hospital), Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.); Ma L ( Department of General Surgery, The Third Affiliated Hospital (Shaanxi Provincial People's Hospital), Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.); Zhu J ( Department of General Surgery, The Third Affiliated Hospital (Shaanxi Provincial People's Hospital), Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.) |
| Abstract | Gastric carcinoma (GC) ranks as the second most common cause of cancerassociated mortality worldwide. Emerging evidence has suggested a potential novel therapeutic strategy based on the ability of cancer stem cells (CSCs) to trigger tumorigenesis. MicroRNAs (miRNAs) have previously been implicated in CSC formation and regulation of their functional characteristics. In the current study, a significant upregulation of miR4835p levels was demonstrated in spheroid bodyforming cells (P<0.01) by reverse transcriptionquantitative polymerase chain reaction, which were isolated from the MKN45 gastric cancer cell line and possessed gastric CSC (GCSC) properties. An MTT assay demonstrated that overexpression of miR4835p by transfection with miR4835p mimics significantly increased cell proliferation and Annexin Vpropidium iodide staining indicated the suppression of cell apoptosis, suggesting that miR4835p has an important function in GCSC growth. Notably, Transwell and sphere formation assays demonstrated that miR4835p elevation promoted GCSC invasion and cell selfrenewal ability, respectively. Further western blotting assays demonstrated that miR4835p upregulation induced an increase in the protein expression levels of ßcatenin and its downstream target molecules, including cyclin D1, Bcl2 and matrix metalloproteinase 2, indicating that miR4835p activates Wnt/ßcatenin signaling. Inhibition of this pathway by ßcatenin small interfering RNA transfection attenuated the miR4835pinduced effects on cell growth, invasion and selfrenewal. These results demonstrate that miR4835p may act as an oncogene to promote the development of GC by regulating GCSC growth, invasion and selfrenewal via the Wnt/ßcatenin signaling pathway. Thus, the present study suggests that miR4835p may be a promising therapeutic target against GC. |
| ISSN | 17912997 |
| e-ISSN | 17913004 |
| Journal | Molecular Medicine Reports |
| Issue Number | 4 |
| Volume Number | 14 |
| Language | English |
| Publisher | Spandidos Publications |
| Publisher Date | 2016-10-01 |
| Publisher Place | Greece |
| Access Restriction | Open |
| Subject Keyword | Discipline Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
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