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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fan, You-Fei Wang, Yu-Lin Hou, Xiao-Ming Jin, Yu-Ting Chen, Xing |
| Description | Author Affiliation: Chen X ( Department of Pediatrics, Provincial Hospital Afï¬ liated to Shandong University, Jinan, Shandong 250021, P.R. China.); Hou XM ( Department of Pediatrics, Provincial Hospital Afï¬ liated to Shandong University, Jinan, Shandong 250021, P.R. China.); Fan YF ( Department of Pediatrics, Provincial Hospital Afï¬ liated to Shandong University, Jinan, Shandong 250021, P.R. China.); Jin YT ( Department of Pediatrics, Provincial Hospital Afï¬ liated to Shandong University, Jinan, Shandong 250021, P.R. China.); Wang YL ( Department of Pediatrics, Provincial Hospital Afï¬ liated to Shandong University, Jinan, Shandong 250021, P.R. China.) |
| Abstract | The sonic hedgehog (SHH) morphogen regulates cell differentiation and controls a number of genes during renal morphogenesis. To date, the effects of SHH on fibroblast growth factors (Fgfs) in embryonic kidney development remain unclear. In the present study, explants of BALB/c mouse embryonic kidney tissues were used to investigate the role of exogenous SHH on Fgf8 and Fgf10 expression levels ex vivo. Ureteric bud branches and epithelial metanephric derivatives were used to determine the renal morphogenesis with Dolichos biflorus agglutinin or hematoxylineosin staining. mRNA expression levels were determined using reverse transcriptionquantitative polymerase chain reaction, while the protein expression levels were examined using immunohistochemistry and western blot analysis. During the initial stages of metanephric development, low levels of SHH, Fgf8, and Fgf10 expression were observed, which were found to increase significantly during more advanced stages of metanephric development. In addition, exogenous SHH protein treatment increased the number of ureteric bud branches and enhanced the formation of nephrons. Exogenous SHH reduced the Fgf8 mRNA and protein expression levels, whereas cyclopamine (an SHHsmoothened receptor inhibitor) interfered with SHHmediated downregulation of Fgf8 expression. By contrast, exogenous SHH protein was not found to modulate Fgf10 mRNA and protein expression levels. In conclusion, these results indicate that the modulatory effects of SHH on BALB/c mouse metanephric explant cultures may involve the regulation of Fgf8 expression but not Fgf10 expression, which provides evidence for the functional role of Fgf proteins in renal morphogenesis. |
| ISSN | 17912997 |
| e-ISSN | 17913004 |
| Journal | Molecular Medicine Reports |
| Issue Number | 4 |
| Volume Number | 14 |
| Language | English |
| Publisher | Spandidos Publications |
| Publisher Date | 2016-10-01 |
| Publisher Place | Greece |
| Access Restriction | Open |
| Subject Keyword | Discipline Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
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