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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bangar, Mangesh A. Muñoz, Francisco Javier Baldrich, Eva García-Aljaro, Cristina Mulchandani, Ashok |
| Description | Country affiliation: Spain Author Affiliation: García-Aljaro C ( Institut de Microelectrónica de Barcelona (CNM-IMB), CSIC, Esfera UAB, Campus UAB, 08193 Bellaterra, Barcelona, Spain.) |
| Abstract | Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r(2)=0.992) for low spore concentrations ranging from 1 to 100 CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30 min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. |
| ISSN | 09565663 |
| Issue Number | 10 |
| Volume Number | 25 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-06-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacillus Isolation & Purification Biosensing Techniques Instrumentation Conductometry Escherichia Coli Nanostructures Chemistry Polymers Spores, Bacterial Electric Impedance Equipment Design Equipment Failure Analysis Ultrastructure Nanotechnology Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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