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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kong, Rong-Mei Shen, Guo-Li Yu, Ru-Qin Liu, Ya-Ru Zhang, Xiao-Bing Hu, Rong Donovan, Michael J. Tan, Weihong |
| Description | Country affiliation: China Author Affiliation: Hu R ( Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, China.) |
| Abstract | The double-strand DNA (dsDNA) can act as an efficient template for the formation of copper nanoparticles (Cu NPs) with high fluorescence, whereas the single-strand DNA (ssDNA) cannot support the formation of Cu NPs. This difference in fluorescent signal generation can be used for the detection of nuclease cleavage activity. Thus, a label-free strategy for sensitive detection of nuclease has been developed. The sensor contains a complete complementary dsDNA which acts as a template for the formation of Cu NPs and generation of fluorescence signal. The enzyme S1 nuclease was taken as the model analyte. Upon addition of S1 nuclease into the sensing system, the DNA was cleaved into fragments, preventing the formation of the Cu NPs and resulting in low fluorescence. In order to achieve the system's best sensing performance, a series of experimental conditions were optimized. Under the optimized experimental conditions, the sensor exhibits excellent performance (e.g., a detection limit of 0.3 U mLâ »¹ with high selectivity). This possibly makes it an attractive platform for the detection of S1 nuclease and other biomolecules. |
| ISSN | 09565663 |
| Volume Number | 42 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Copper Chemistry Dna Deoxyribonucleases Isolation & Purification Metal Nanoparticles Biosensing Techniques Methods Fluorescent Dyes Humans Spectrometry, Fluorescence Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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