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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yung, Ken K. L. Chan, W. S. Mo, Z. X. Hung, Karen W. Y. Chen, Y. F. Suen, Miranda F. K. |
| Description | Country affiliation: Hong Kong Author Affiliation: Suen MF ( Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.) |
| Abstract | Transgenic zebrafish are a common vertebrate model system for the study of addictive behavior. In the present study, plasmid constructs containing green fluorescent protein (GFP) and the promoter of tyrosine hydroxylase (TH), a key synthetic enzyme for catecholamines, were produced. The TH-GFP constructs were microinjected into zebrafish embryonic cells. Three days post-fertilization, GFP began expressing in distinct catecholaminergic areas. The TH-GFP transgenic zebrafish were employed as live biosensors to test the effects of the commonly abused drugs nicotine and ketamine. First, locomotion assays were used to study the general excitatory effects of the drugs. Maximal locomotor activity was obtained after treatment with a high concentration of nicotine (10 µM), but with a much lower concentration of ketamine (0.1 µM). Second, TH protein levels in zebrafish brains were assessed by Western blot. TH protein levels were significantly increased, with maximal protein levels found after treatment with the same drug concentrations that gave maximal locomotor activity. Importantly, analysis of GFP in the zebrafish catecholaminergic areas revealed the same expression patterns as was obtained by Western blot. The present results indicate that increased locomotor activity can be correlated to TH protein expression, as indicated by Western blot and expression of TH-GFP. We have shown that TH-GFP expression is a reliable method to show the effects of drugs on TH expression that may be employed as a novel high-throughput live biosensor for screening drugs of abuse. |
| ISSN | 09565663 |
| Volume Number | 42 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Green Fluorescent Proteins Chemistry Locomotion Drug Effects Tyrosine 3-monooxygenase Animals Animals, Genetically Modified Ketamine Administration & Dosage Nicotine Promoter Regions, Genetic Street Drugs Zebrafish Embryology Genetics Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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