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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, Guiyan Fang, Xuexun Li, Haolong Fang, Xinxiu Xu, Jingwei Yang, Wei |
| Description | Author Affiliation: Fang X ( State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.) |
| Abstract | Three chemosensors for Zn²â º were designed and synthesized based on the chromophore of the blue fluorescent protein (BFP). Among them, IMMPI (4-((1H-imidazol-2-yl)methylene)-1-methyl-2-phenyl-1H-imidazol-5(4H)-one) contained one BFP chromophore unit, which sequestered Zn²â º with nitrogen in the two imidazole rings, while Di-IMMPI-a and Di-IMMPI-o contained two IMMPI units that were connected together using an alkane and a methoxy chain, respectively. All three molecules selectively interacted with Zn²â º with a 1:1 mode. Di-IMMPI-a had a dissociation constant <0.03 µM for Zn²â º, more than 30 folds stronger than that of IMMPI. The fluorescences of IMMPI, Di-IMMPI-a, and Di-IMMPI-o were turned on upon the binding of Zn²â º, accompanied by the quantum yields enhancing from <0.002 to 0.352, 0.451, and 0.194, respectively. Fluorescence images showed that Di-IMMPI-a responded to Zn²â º in cell cytosol. Our work demonstrated the potentiality for the development of practical Zn²â º sensors based on the isolated BFP chromophores without the proteinframe. |
| ISSN | 09565663 |
| Volume Number | 42 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Ions Isolation & Purification Luminescent Proteins Chemistry Zinc Fluorescence Green Fluorescent Proteins Hela Cells Humans Imidazoles Spectrometry, Fluorescence Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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