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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lin, Jianhan Xiong, Yonghua Wang, Dan Chen, Qi Gan, Chengqi Wang, Yuhe Wang, Maohua Lai, Weihua Li, Yuntao |
| Description | Country affiliation: China Author Affiliation: Chen Q ( MOA Key Laboratory of Agricultural Information Acquisition Technology (Beijing), China Agricultural University, Beijing, China.); Lin J ( MOA Key Laboratory of Agricultural Information Acquisition Technology (Beijing), China Agricultural University, Beijing, China. Electronic address: jianhan@cau.edu.cn.); Gan C ( MOA Key Laboratory of Agricultural Information Acquisition Technology (Beijing), China Agricultural University, Beijing, China.); Wang Y ( MOA Key Laboratory of Agricultural Information Acquisition Technology (Beijing), China Agricultural University, Beijing, China.); Wang D ( MOA Key Laboratory of Agricultural Information Acquisition Technology (Beijing), China Agricultural University, Beijing, China.); Xiong Y ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Lai W ( State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.); Li Y ( State Key Lab of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Science, Beijing, China.); Wang M ( Modern Precision Agriculture System Integration Research Key Laboratory of Ministry of Education, China Agricultural University, Beijing, China.) |
| Abstract | In this study, we described a novel impedance biosensor combining immunomagnetic separation with urease catalysis for sensitive detection of foodborne bacteria using Listeria monocytogenes as model and an immobilization-free microelectrode as detector. The monoclonal antibodies (MAbs) were immobilized on the surface of the magnetic nanoparticles (MNPs) with the diameter of 180 nm by biotin-streptavidin system for specifically and efficiently separating Listeria cells from sample background. The polyclonal antibodies (PAbs) and the urease were modified onto the surface of the gold nanoparticles (AuNPs) with the diameter of 20 nm and the modified AuNPs were used to react with Listera to form the MNP-MAb-Listeria-PAb-AuNP-urease sandwich complexes. The urease in the complexes could catalyze the hydrolysis of the urea into ammonium carbonate and this led to an increase in the ionic strength of the media, which could be detected by the microelectrode. The magnetic separation efficiencies for L. monocytogenes at the concentrations ranging from 3.0×10(1) to 3.0×10(4) CFU/mL were over 95% for the pure cultures and over 85% for the spiked lettuce samples. The lower detection limit of this biosensor for L. monocytogenes was found to be 300 CFU/mL in both the pure cultures and the spiked lettuce samples. The microelectrode was demonstrated to be reusable for over 50 times with thorough cleaning by deionized water. This biosensor showed its potential to provide a simple, low-cost and sensitive method for rapid screening of foodborne pathogens and could be extended for detection of other biological or chemical targets. |
| ISSN | 09565663 |
| Volume Number | 74 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Conductometry Food Analysis Immunomagnetic Separation Listeria Monocytogenes Isolation & Purification Urease Chemistry Bacterial Load Catalysis Equipment Design Equipment Failure Analysis Food Contamination Analysis Food Microbiology Microelectrodes Tissue Array Analysis Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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