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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nakamura, M. Horii, J. Nishimoto, T. Masuda, H. Kuma, K. I. Nishitani, H. Yokoyama, N. Ohba, T. Tanaka, M. Miyata, T. |
| Description | Author Affiliation: Nakamura M ( Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812-82, Japan.) |
| Abstract | A novel human protein with a molecular mass of 55 kD, designated RanBPM, was isolated with the two-hybrid method using Ran as a bait. Mouse and hamster RanBPM possessed a polypeptide identical to the human one. Furthermore, Saccharomyces cerevisiae was found to have a gene, YGL227w, the COOH-terminal half of which is 30% identical to RanBPM. Anti-RanBPM antibodies revealed that RanBPM was localized within the centrosome throughout the cell cycle. Overexpression of RanBPM produced multiple spots which were colocalized with γ-tubulin and acted as ectopic microtubule nucleation sites, resulting in a reorganization of microtubule network. RanBPM cosedimented with the centrosomal fractions by sucrose- density gradient centrifugation. The formation of microtubule asters was inhibited not only by anti- RanBPM antibodies, but also by nonhydrolyzable GTP-Ran. Indeed, RanBPM specifically interacted with GTP-Ran in two-hybrid assay. The central part of asters stained by anti-RanBPM antibodies or by the mAb to γ-tubulin was faded by the addition of GTPγS-Ran, but not by the addition of anti-RanBPM anti- bodies. These results provide evidence that the Ran-binding protein, RanBPM, is involved in microtubule nucleation, thereby suggesting that Ran regulates the centrosome through RanBPM. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 143 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1998-11-16 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Centrosome Chemistry GTP-Binding Proteins Genetics Microtubules Nuclear Proteins Tubulin Ran GTP-Binding Protein Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals CHO Cells COS Cells Drug Effects Cricetinae Cytoskeletal Proteins DNA, Complementary Evolution, Molecular Gene Expression Physiology Guanosine 5'-O-(3-Thiotriphosphate) Pharmacology HeLa Cells Immunoblotting Molecular Sequence Data Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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