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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Salamero, J. Tenza, D. Johannes, L. Goud, B. Mallard, F. Antony, C. |
| Description | Author Affiliation: Mallard F ( Institut Curie, Centre National de la Recherche Scientifique UMR 144, Laboratoire Mécanismes Moléculaires du Transport Intracellulaire, F-75248 Paris Cedex 05, France.) |
| Abstract | Shiga toxin and other toxins of this family can escape the endocytic pathway and reach the Golgi apparatus. To synchronize endosome to Golgi transport, Shiga toxin B-fragment was internalized into HeLa cells at low temperatures. Under these conditions, the protein partitioned away from markers destined for the late endocytic pathway and colocalized extensively with cointernalized transferrin. Upon subsequent incubation at 37°C, ultrastructural studies on cryosections failed to detect B-fragment–specific label in multivesicular or multilamellar late endosomes, suggesting that the protein bypassed the late endocytic pathway on its way to the Golgi apparatus. This hypothesis was further supported by the rapid kinetics of B-fragment transport, as determined by quantitative confocal microscopy on living cells and by B-fragment sulfation analysis, and by the observation that actin- depolymerizing and pH-neutralizing drugs that modulate vesicular transport in the late endocytic pathway had no effect on B-fragment accumulation in the Golgi apparatus. B-fragment sorting at the level of early/recycling endosomes seemed to involve vesicular coats, since brefeldin A treatment led to B-fragment accumulation in transferrin receptor–containing membrane tubules, and since B-fragment colocalized with adaptor protein type 1 clathrin coat components on early/recycling endosomes. Thus, we hypothesize that Shiga toxin B-fragment is transported directly from early/recycling endosomes to the Golgi apparatus. This pathway may also be used by cellular proteins, as deduced from our finding that TGN38 colocalized with the B-fragment on its transport from the plasma membrane to the TGN. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 143 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1998-11-16 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Toxins Pharmacokinetics Endosomes Metabolism Golgi Apparatus Adaptor Protein Complex Gamma Subunits Biological Transport Drug Effects Physiology Brefeldin A Pharmacology Cell Fractionation Cell Membrane Ultrastructure Clathrin Exotoxins HeLa Cells Kinetics Membrane Proteins Microscopy, Immunoelectron Oligopeptides Peptide Fragments Protein Sorting Signals Protein Synthesis Inhibitors Shiga Toxins Temperature Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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