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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Brandt, Sonja Von Stetten, David Hildebrandt, Peter Frankenberg-dinkel, Nicole Günther, Mina |
| Description | Author Affiliation: Brandt S ( Physiologie der Mikroorganismen, Ruhr-Universität Bochum, Universitätsstrasse 150, Bochum D-44780, Germany.) |
| Abstract | The red light-sensing photoreceptor FphA from Aspergillus nidulans is involved in the regulation of developmental processes in response to light. Here we present extended biochemical and spectroscopic characterization of recombinant FphA using a synthetic gene with host-adapted codon usage. The recombinant photosensory domain FphAN753 was shown to display all features of a bona fide phytochrome. It covalently binds biliverdin as chromophore and undergoes red/far-red light-inducible photoconversion with both parent states being protonated. The large N-terminal variable extension of FphA exerts a stabilizing effect on the active Pfr state. Upon substitution of the highly conserved histidine 504, involved in the hydrogen-bonding network of the protein moiety and the chromophore, chromophore attachment and photoreversibility were completely impaired. FphA is a functional sensor histidine kinase with a strong red-light-dependent autophosphorylation activity. Furthermore, intermolecular trans-phosphorylation to the response regulator domain of a second monomer could be demonstrated. Interestingly, co-incubation of FphA and FphA variants led to enhanced autophosphorylation, including the 'inactive' Pr form. The latter observed phenomenon might suggest that auto- and trans-phosphorylation activity is modulated by additional interaction partners leading to variable phosphorylation events that trigger a specific output response. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 50 |
| Volume Number | 283 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2008-12-12 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Aspergillus Nidulans Metabolism Phycobiliproteins Chemistry Phytochrome Biliverdine Deinococcus Histidine Kinetics Models, Chemical Molecular Sequence Data Phosphorylation Physiology Protein Structure, Tertiary Recombinant Proteins Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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