NDLI: The inhibitory mechanism of protein synthesis by YoeB, an Escherichia coli toxin.

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The inhibitory mechanism of protein synthesis by YoeB, an Escherichia coli toxin.

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The inhibitory mechanism of protein synthesis by YoeB, an Escherichia coli toxin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Inouye, Masayori Zhang, Yonglong
Description	Author Affiliation: Zhang Y ( Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.)
Abstract	YoeB is a toxin encoded by the yefM-yoeB antitoxin-toxin operon in the Escherichia coli genome. Here we show that YoeB, a highly potent protein synthesis inhibitor, specifically blocks translation initiation. In in vivo primer extension experiments using two different mRNAs, a major band was detected after YoeB induction at three bases downstream of the initiation codon at 2.5 min. An identical band was also detected in in vitro toeprinting experiments after the addition of YoeB to the reaction mixtures containing 70 S ribosomes and the same mRNAs, even in the absence of tRNA(f)(Met). Notably, this band was not detected in the presence of YoeB alone, indicating that YoeB by itself does not have endoribonuclease activity under the conditions used. The 70 S ribosomes increased upon YoeB induction, and YoeB was found to be specifically associated with 50 S subunits. Using tetracycline and hygromycin B, we demonstrated that YoeB binds to the 50 S ribosomal subunit in 70 S ribosomes and interacts with the A site leading to mRNA cleavage at this site. As a result, the 3'-end portion of the mRNA was released from ribosomes, and translation initiation was effectively inhibited. These results demonstrate that YoeB primarily inhibits translation initiation.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	11
Volume Number	284
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2009-03-13
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Toxins Metabolism Codon, Initiator Escherichia Coli Proteins Escherichia Coli Peptide Chain Initiation, Translational Physiology Protein Synthesis Inhibitors RNA, Bacterial Anti-Bacterial Agents Pharmacology Chemistry Cell-Free System Hygromycin B Drug Effects RNA, Transfer, Amino Acyl RNA, Transfer, Met Ribosome Subunits, Large, Bacterial Tetracycline Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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