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DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.

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Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

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Growth cone formation in cultures of sensory neurons.

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Growth cone formation in cultures of sensory neurons.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Thomas, C. Bray, D. Shaw, G.
Abstract	Three experimental situations have been found in which cultured sensory neurons from embryonic chicken will form growth cones from positions along the length of the neurite. If the neurons are dissected with a remaining short axonal stump and plated into serum-free medium, they can form a morphologically normal growth cone from the stump within 15 min, even in the presence of cycloheximide or puromycin. When neurites growing in culture media with low levels of serum are cut at any point with microneedles, growth cones are produced quickly from the amputated stump, usually within 20 min. Treatment of growing neurons with low concentrations of colchicine, Colcemid, or podophyllotoxin results in the progressive appearance of lateral filopodia and regions of flattened cytoplasm that closely resemble growth cones except for their preterminal positions. These observations show that the potential to form growth cones is distributed throughout the neuron and suggest that this normally repressed in some way by the neuronal microtubules.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	75
Language	English
Publisher	National Academy of Sciences
Publisher Date	1978-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Neurons Physiology Axons Cells, Cultured Colchicine Pharmacology Cycloheximide Cytochalasin B Demecolcine Ganglia, Spinal Cytology Nerve Tissue Proteins Biosynthesis Podophyllotoxin Puromycin Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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