NDLI: Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.

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Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Teem, J. L. Rosbash, M.
Abstract	The temperature-sensitive mutation rna2 causes the accumulation of higher molecular weight transcripts from the ribosomal protein 51 (rp51) gene of yeast and many other yeast ribosomal protein genes. We have determined the DNA sequence of the rp51 gene, confirming that it contains an intron and that the higher molecular weight transcript is an intron-containing precursor RNA. These data and other experiments suggest that the rna2 mutation affects mRNA processing (splicing) and that the presence of an intron is sufficient to render expression of a gene sensitive to the rna2 mutation. To test these hypotheses, we have inserted the rp51 intron into the coding region of a hybrid Escherichia coli beta-galactosidase gene, thereby interrupting the open reading frame subsequent to the initiating methionine codon. Despite the presence of the intron, the beta-galactosidase gene is expressed in yeast. Thus, the rp51 intron is properly excised from the normally intronless gene. The presence of the rp51 intron causes the beta-galactosidase activity to be sensitive to the rna2 mutation, consistent with the notion that this mutation affects gene expression at the level of splicing. The experiments suggest that an intron-containing beta-galactosidase gene can be used in a general way to study mRNA splicing.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Galactosidases Genetics Genes Mutation Ribosomal Proteins Saccharomyces Cerevisiae Beta-Galactosidase Amino Acid Sequence Cloning, Molecular DNA Metabolism DNA Restriction Enzymes Plasmids Enzymology Temperature Transcription, Genetic Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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