NDLI: In vitro assembly of a pre-messenger ribonucleoprotein.

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Role of microtubules in the distribution of the Golgi apparatus: effect of taxol and microinjected anti-alpha-tubulin antibodies.

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Selection of functional cDNAs by complementation in yeast.

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

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In vitro synthesis of bacteriophage phi X174 by purified components.

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Molecular cloning of a vitamin D-dependent calcium-binding protein mRNA sequence from chick intestine.

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Adenovirus DNA replication in vitro: synthesis of full-length DNA with purified proteins.

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In vitro assembly of a pre-messenger ribonucleoprotein.

Genomic environment of variant surface antigen genes of Trypanosoma equiperdum.

Gating of ion channels made by a diphtheria toxin fragment in phospholipid bilayer membranes.

Visualization of antigens attached to cytoskeletal framework in animal cells: colocalization of simian virus 40 Vp1 polypeptide and actin in TC7 cells.

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Molecular cloning and characterization of the gene coding for human complement protein factor B.

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Steady-state kinetic study of fatty acid synthase from chicken liver.

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Molecular cloning of the cDNA coding for rat ornithine transcarbamoylase.

Detection of a guanine X adenine base pair in a decadeoxyribonucleotide by proton magnetic resonance spectroscopy.

Growth-related changes in specific mRNAs of cultured mouse cells.

Activation of calcineurin by limited proteolysis.

Computer simulation of the dynamics of hydrated protein crystals and its comparison with x-ray data.

New view of lipid bilayer dynamics from 2H and 13C NMR relaxation time measurements.

Phorbol ester inhibits furosemide-sensitive potassium transport in BALB/c 3T3 preadipose cells.

Immunocytochemical localization of alpha-D-mannosidase II in the Golgi apparatus of rat liver.

Comparison of secretory protein and membrane composition of secretory granules isolated from normal and neoplastic pancreatic acinar cells of rats.

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Trans-acting temporal locus within the beta-glucuronidase gene complex.

Chromosomal assignment of a family of human oncogenes.

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Evidence for the general base mechanism in carboxypeptidase A-catalyzed reactions: partitioning studies on nucleophiles and H2(18)O kinetic isotope effects.

Identification of the molecular defect in factor IX Chapel Hill: substitution of histidine for arginine at position 145.

Naturally occurring cytotoxic human antibodies recognize H-2-controlled murine lymphocyte antigens.

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In vitro assembly of a pre-messenger ribonucleoprotein.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Economidis, I. V. Pederson, T.
Abstract	Transcription of the Bal I E restriction fragment of adenovirus DNA by RNA polymerase II in a HeLa cell extract produces a RNA transcript 1,712 nucleotides in length. This transcript contains the first two elements of the tripartite leader that, in vivo, is spliced onto the late mRNAs. We have found that this adenovirus 2 transcript forms a specific ribonucleoprotein complex (RNP) in this in vitro system. The RNP particle sediments in sucrose gradients as a monodisperse peak at 50 S and has a buoyant density of 1.34 g/cm3 in Cs2SO4, indicating the same 4:1 protein/RNA composition as native nuclear RNPs that contain pre-mRNA sequences (hnRNP). Moreover, the in vitro-assembled RNP is resistant to concentrations of NaCl that are known to dissociate nonspecific RNA-protein complexes. The adenovirus 2 transcript is precipitated by a monoclonal antibody for hnRNP core proteins. In addition, RNA-protein crosslinking of [alpha-32P]UTP-labeled transcript/RNP complexes reveals that the major proteins in contact with the RNA are the Mr 32,500-41,500 species known to be associated with hnRNA in vivo. These results demonstrate the in vitro assembly of a specific RNA polymerase II transcript into RNP. Moreover, because the 1,712-nucleotide adenovirus 2 transcript lacks poly(A) addition sites and because the leader sequences are not spliced appreciably in this in vitro system, it follows that RNP formation requires neither polyadenylylation nor splicing, nor is it sufficient to cause the latter.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adenoviruses, Human Genetics DNA-Directed RNA Polymerases Metabolism Nucleoproteins Protein Precursors RNA Polymerase II Ribonucleoproteins Transcription, Genetic DNA Restriction Enzymes DNA, Viral Electrophoresis, Polyacrylamide Gel HeLa Cells Enzymology RNA, Heterogeneous Nuclear Viral Proteins Isolation & Purification Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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